Lysyl oxidase propeptide stimulates osteoblast and osteoclast differentiation in the presence or absence of prostate cancer cell-derived factors
Alsulaiman, Mona Abdulaziz W.
MetadataShow full item record
BACKGROUND AND OBJECTIVES : Lysyl oxida se propeptide is secreted as a 50 kDa pro-enzyme into extracellular matrix where it is cleaved into 30-kDa mature enzyme (LOX) and 18 kDa pro-peptide (LOX-PP) . The role of LOX enzyme in extracellular matrix maturation has been extensively studied and linked to cross-linking of collagen and elastin molecules. Previous studies have indicated that LOX-PP acts as a tumor suppressor by observing its effect on transformed and malignant cell lines. Here we investigate the effect of LOX-PP on stromal cell proliferation and differentiation towards osteoblasts or osteoclasts and its interference with prostate cancer cell induced alterations on proliferation and differentiation of bone marrow stromal cells. MATERIALS AND METHODS: Bone marrow stromal cell cultures were generated from the femur and tibia of adult 8-10 week old CD- I male mice. Cells were treated with rLOX-PP or prostate cancer conditioned medium in the presence and absence of rLOXPP. Lysozyme was used in the control as well as the treatment group and used as a [TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Dissertation (DScD) --Boston University, Henry M. Goldman School of Dental Medicine, 2015 (Department of Molecular and Cell Biology).Includes bibliography: leaves 219-233.
RightsThis work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact email@example.com.