Lysyl oxidase propeptide stimulates osteoblast and osteoclast differentiation in the presence or absence of prostate cancer cell-derived factors
Alsulaiman, Mona Abdulaziz W.
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BACKGROUND AND OBJECTIVES : Lysyl oxida se propeptide is secreted as a 50 kDa pro-enzyme into extracellular matrix where it is cleaved into 30-kDa mature enzyme (LOX) and 18 kDa pro-peptide (LOX-PP) . The role of LOX enzyme in extracellular matrix maturation has been extensively studied and linked to cross-linking of collagen and elastin molecules. Previous studies have indicated that LOX-PP acts as a tumor suppressor by observing its effect on transformed and malignant cell lines. Here we investigate the effect of LOX-PP on stromal cell proliferation and differentiation towards osteoblasts or osteoclasts and its interference with prostate cancer cell induced alterations on proliferation and differentiation of bone marrow stromal cells. MATERIALS AND METHODS: Bone marrow stromal cell cultures were generated from the femur and tibia of adult 8-10 week old CD- I male mice. Cells were treated with rLOX-PP or prostate cancer conditioned medium in the presence and absence of rLOXPP. Lysozyme was used in the control as well as the treatment group and used as a [TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Dissertation (DScD) --Boston University, Henry M. Goldman School of Dental Medicine, 2015 (Department of Molecular and Cell Biology).Includes bibliography: leaves 219-233.
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