Characterization of the lactate permease operon of Veillonellae Atypica PK 1910
Babae, Raed Ahmed
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Veillonellae have been extensively studied and have been found to have an unusual metabolism. They do not metabolize carbohydrates such as glucose as an energy source for growth, instead, they can grow well anaerobically in the presence of nonfermentable substrates. It has been proposed that Vel/onellae play an important role in reducing the cariogenic potential of plague bacteria. From a previous study, three ORFs were identified in let permease operon of V. atypica PK 1910: ORFl (mdh) of 1.136 hp, ORF2 (lctP) of 1.641 bp, and ORF3 (hem) of 1.257 bp. In this study we focused on the expression and regulation of lctP operon in V. atypica PK 1910 by environmental conditions. The bacteria was grown anaerobically in Scaedler broth supplemented with different acids: lactate, pyruvate, malate, formate, fumarate, and succinate. The growth was significantly better with lactate or pyruvate containing media. RNA purification was successful from the bacteria grown in different media in all different growth phases (logarithmic phase, stationary phase, and overnight culture) examined. [TRUNCATED]
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