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dc.contributor.authorBabae, Raed Ahmeden_US
dc.date.accessioned2018-10-31T15:41:59Z
dc.date.available2018-10-31T15:41:59Z
dc.date.issued2002
dc.date.submitted2002
dc.identifier.otherb24504191
dc.identifier.other(OCoLC)50923541
dc.identifier.urihttps://hdl.handle.net/2144/31732
dc.descriptionPLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.descriptionThesis (D.Sc.D.)--Boston University Henry M. Goldman School of Dental Medicine, 2002 (Pediatric Dentistry).en_US
dc.description.abstractVeillonellae have been extensively studied and have been found to have an unusual metabolism. They do not metabolize carbohydrates such as glucose as an energy source for growth, instead, they can grow well anaerobically in the presence of nonfermentable substrates. It has been proposed that Vel/onellae play an important role in reducing the cariogenic potential of plague bacteria. From a previous study, three ORFs were identified in let permease operon of V. atypica PK 1910: ORFl (mdh) of 1.136 hp, ORF2 (lctP) of 1.641 bp, and ORF3 (hem) of 1.257 bp. In this study we focused on the expression and regulation of lctP operon in V. atypica PK 1910 by environmental conditions. The bacteria was grown anaerobically in Scaedler broth supplemented with different acids: lactate, pyruvate, malate, formate, fumarate, and succinate. The growth was significantly better with lactate or pyruvate containing media. RNA purification was successful from the bacteria grown in different media in all different growth phases (logarithmic phase, stationary phase, and overnight culture) examined. [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.subjectLactic aciden_US
dc.subjectVeillonellaen_US
dc.titleCharacterization of the lactate permease operon of Veillonellae Atypica PK 1910en_US
dc.typeThesis/Dissertationen_US
etd.degree.nameDoctor of Science in Dentistryen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplinePediatric Dentistryen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.mmsid99187400030001161


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