Molecular characterization and functional properties of human salivary mucins MG1 and MG2
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Nine human mucin genes have been identified to date and these have been named MUCl-4 , MUC5AC, MUC5B and MUC6-8 . Human submandibular/sublingual gland secretions contain both a high molecular weight mucin, MG 1 and a low molecular weight mucin, MG2. The MUC5B gene product is a major component of MG 1 and MUC7 is a single copy gene encoding MG2. MG 1 has been suggested to consist of a mixture of mucin gene products and a major goal of this study was to identify other mucin genes comprising MG 1. Polyclonal antibodies against deglycosylated MG 1 were used to screen human submandibular and sublingual gland cDNA libraries and, after screening 200,000 pfus, no clones corresponding to previously unidentified mucins were found in either library. Since previous Northern blotting studies indicated the presence of small quantities of MUC4 mRNAs in submandibular gland, both libraries were screened with a MUC4 DNA probe. Two clones containing MUC4 tandem repeats were isolated from the submandibular gland library. A recombinant MUC4 protein was expressed in Escherichia coli and this protein was found to react with polyclonal antibodies against deglycosylated MG 1. These studies show conclusively that MG 1 contains both MUC5B and MUC4 gene products suggesting that each mucin may perform distinct functions in the oral cavity. [TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Periodontology and Oral Biology).Includes bibliographical references (leaves 176-194).
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