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    • ENG: Bioinformatics: Scholarly Papers
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    •   OpenBU
    • College of Engineering
    • Bioinformatics
    • ENG: Bioinformatics: Scholarly Papers
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    Systematic Variation in mRNA 3′-Processing Signals during Mouse Spermatogenesis

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    Date Issued
    2006-12-08
    Publisher Version
    10.1093/nar/gkl919
    Author(s)
    Liu, Donglin
    Brockman, J. Michael
    Dass, Brinda
    Hutchins, Lucie N.
    Singh, Priyam
    McCarrey, John R.
    MacDonald, Clinton C.
    Graber, Joel H.
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    Permanent Link
    https://hdl.handle.net/2144/3205
    Citation (published version)
    Liu, Donglin, J. Michael Brockman, Brinda Dass, Lucie N. Hutchins, Priyam Singh, John R. McCarrey, Clinton C. MacDonald, Joel H. Graber. "Systematic variation in mRNA 3′-processing signals during mouse spermatogenesis" Nucleic Acids Research 35(1): 234-246. (2006)
    Abstract
    Gene expression and processing during mouse male germ cell maturation (spermatogenesis) is highly specialized. Previous reports have suggested that there is a high incidence of alternative 3′-processing in male germ cell mRNAs, including reduced usage of the canonical polyadenylation signal, AAUAAA. We used EST libraries generated from mouse testicular cells to identify 3′-processing sites used at various stages of spermatogenesis (spermatogonia, spermatocytes and round spermatids) and testicular somatic Sertoli cells. We assessed differences in 3′-processing characteristics in the testicular samples, compared to control sets of widely used 3′-processing sites. Using a new method for comparison of degenerate regulatory elements between sequence samples, we identified significant changes in the use of putative 3′-processing regulatory sequence elements in all spermatogenic cell types. In addition, we observed a trend towards truncated 3′-untranslated regions (3′-UTRs), with the most significant differences apparent in round spermatids. In contrast, Sertoli cells displayed a much smaller trend towards 3′-UTR truncation and no significant difference in 3′-processing regulatory sequences. Finally, we identified a number of genes encoding mRNAs that were specifically subject to alternative 3′-processing during meiosis and postmeiotic development. Our results highlight developmental differences in polyadenylation site choice and in the elements that likely control them during spermatogenesis.
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    • ENG: Bioinformatics: Scholarly Papers [101]


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