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    • School of Medicine
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    • Department of Biochemistry
    • MED: Biochemistry Papers
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    •   OpenBU
    • School of Medicine
    • Basic Science
    • Department of Biochemistry
    • MED: Biochemistry Papers
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    Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency

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    Date Issued
    2008-1-16
    Publisher Version
    10.1371/journal.pone.0001417
    Author(s)
    Ilyinskii, Petr O.
    Gambaryan, Alexandra S.
    Meriin, Anatoli B.
    Gabai, Vladimir
    Kartashov, Alex
    Thoidis, Galini
    Shneider, Alexander M.
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    Permanent Link
    https://hdl.handle.net/2144/3227
    Citation (published version)
    Ilyinskii, Petr O., Alexandra S. Gambaryan, Anatoli B. Meriin, Vladimir Gabai, Alex Kartashov, Galini Thoidis, Alexander M. Shneider. "Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency" PLoS ONE3(1): 1417. (2008)
    Abstract
    The effectiveness of recombinant vaccines encoding full-length M2 protein of influenza virus or its ectodomain (M2e) have previously been tested in a number of models with varying degrees of success. Recently, we reported a strong cytotoxic effect exhibited by M2 on mammalian cells in vitro. Here we demonstrated a decrease in protection when M2 was added to a DNA vaccination regimen that included influenza NP. Furthermore, we have constructed several fusion proteins of conserved genes of influenza virus and tested their expression in vitro and protective potential in vivo. The four-partite NP-M1-M2-NS1 fusion antigen that has M2 sequence engineered in the middle part of the composite protein was shown to not be cytotoxic in vitro. A three-partite fusion protein (consisting of NP, M1 and NS1) was expressed much more efficiently than the four-partite protein. Both of these constructs provided statistically significant protection upon DNA vaccination, with construct NP-M1-M2-NS1 being the most effective. We conclude that incorporation of M2 into a vaccination regimen may be beneficial only when its apparent cytotoxicity-linked negative effects are neutralized. The possible significance of this data for influenza vaccination regimens and preparations is discussed.
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    • MED: Biochemistry Papers [27]


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