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dc.contributor.advisorKandarian, Susan C.en_US
dc.contributor.authorGaney, Johnen_US
dc.date.accessioned2018-11-30T16:33:00Z
dc.date.available2018-11-30T16:33:00Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/2144/32732
dc.description.abstractCachexia is a multifactorial metabolic wasting syndrome that affects a large percentage of cancer patients and results in the involuntary loss of skeletal muscle and adipose tissue. The consequences of this condition include metabolic imbalances and fatigue, which are strongly associated with poor prognosis. While the specific mechanism for skeletal muscle wasting is still undefined, LIF secreted by C26 colon carcinoma cells has recently be found to induce atrophy in treated myotubes. The purpose of this study is to determine the necessity of LIF for inducing atrophy in mouse myotubes by producing a knockout of Lif in C26 cells using CRIPSR-Cas9. Media was collected from these cells and used to treat myotubes. Measurements of myotube diameters were made and atrophy was compared between myotubes that received medium from C26 and C26Lif-/- cells. A dosage of recombinant mouse LIF was also added to LIF-deficient medium in order to determine if LIF alone was sufficient to induce atrophy. At study endpoint, myotubes that were treated with media taken from C26 cells showed significant signs of atrophy compared to myotubes that were treated C26Lif-/- media. LIF was also shown to be sufficient to induce myotube atrophy on its own, with atrophy being rescued in myotubes that received a dosage of LIF added to C26Lif-/- media. These results demonstrate that LIF is required for atrophy to be induced in mouse myotubes treated with media taken from cancer cells, and can do so independent of other secreted factors.en_US
dc.language.isoen_US
dc.subjectPhysiologyen_US
dc.subjectCachexiaen_US
dc.subjectCanceren_US
dc.subjectCellen_US
dc.subjectCRISPRen_US
dc.subjectLIFen_US
dc.subjectMuscleen_US
dc.titleDetermining the role of tumor-derived leukemia inhibitory factor in cancer cachexia using a genetic approachen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2018-10-24T01:01:40Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineSargent College of Health and Rehabilitation Sciencesen_US
etd.degree.grantorBoston Universityen_US


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