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dc.contributor.authorWu, X.en_US
dc.contributor.authorMorgan, Kathleen G.en_US
dc.contributor.authorJones, C. J.en_US
dc.contributor.authorTribe, R. M.en_US
dc.contributor.authorTaggart, M. J.en_US
dc.date.accessioned2018-12-11T19:29:35Z
dc.date.available2018-12-11T19:29:35Z
dc.date.issued2008-08-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000258358300031&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e74115fe3da270499c3d65c9b17d654
dc.identifier.citationX Wu, KG Morgan, CJ Jones, RM Tribe, MJ Taggart. 2008. "Myometrial mechanoadaptation during pregnancy: implications for smooth muscle plasticity and remodelling." Journal Of Cellular And Molecular Medicine, Volume 12, Issue 4, pp. 1360 - 1373 (14). https://doi.org/10.1111/j.1582-4934.2008.00306.x
dc.identifier.issn1582-1838
dc.identifier.urihttps://hdl.handle.net/2144/32913
dc.description.abstractThe smooth muscle of the uterus during pregnancy presents a unique circumstance of physiological mechanotransduction as the tissue remodels in response to stretches imposed by the growing foetus(es), yet the nature of the molecular and functional adaptations remain unresolved. We studied, in myometrium isolated from non‐pregnant (NP) and pregnant mice, the active and passive length–tension curves by myography and the expression and activation by immunoblotting of focal adhesion‐related proteins known in other systems to participate in mechanosensing and mechanotransduction. In situ uterine mass correlated with pup number and weight throughout pregnancy. In vitro myometrial active, and passive, length‐tension curves shifted significantly to the right during pregnancy indicative of altered mechanosensitivity; at term, maximum active tension was generated following 3.94 ± 0.33‐fold stretch beyond slack length compared to 1.91 ± 0.12‐fold for NP mice. Moreover, mechanotransduction was altered during pregnancy as evidenced by the progressive increase in absolute force production at each optimal stretch. Pregnancy was concomitantly associated with an increased expression of the dense plaque‐associated proteins FAK and paxillin, and elevated activation of FAK, paxillin, c‐Src and extracellular signal‐regulated kinase (ERK1/2) which reversed 1 day post‐partum. Electron microscopy revealed close appositioning of neighbouring myometrial cells across a narrow extracellular cleft adjoining plasmalemmal dense plaques. Collectively, these results suggest a physiological basis of myometrial length adaptation, long known to be a property of many smooth muscles, whereupon plasmalemmal dense plaque proteins serve as molecular signalling and structural platforms contributing to functional (contractile) remodelling in response to chronic stretch.en_US
dc.description.sponsorshipHD43054 - NICHD NIH HHS; R01 HD043054 - NICHD NIH HHS; R01 HD043054-05 - NICHD NIH HHS; Wellcome Trust; HL86655 - NHLBI NIH HHS; P01 HL086655 - NHLBI NIH HHS; HL8003 - NHLBI NIH HHSen_US
dc.format.extentp. 1360 - 1373en_US
dc.languageEnglish
dc.publisherWILEY-BLACKWELLen_US
dc.relation.ispartofJournal Of Cellular And Molecular Medicine
dc.rights© 2008 The Authors. Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltden_US
dc.subjectScience & technologyen_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectCell biologyen_US
dc.subjectMedicine, research & experimentalen_US
dc.subjectResearch & experimental medicineen_US
dc.subjectMechanotransductionen_US
dc.subjectSmooth muscle remodellingen_US
dc.subjectDense plaquesen_US
dc.subjectMyometriumen_US
dc.subjectSmooth muscle plasticityen_US
dc.subjectActinsen_US
dc.subjectAdaptation, physiologicalen_US
dc.subjectAnimalsen_US
dc.subjectBiomechanical phenomenaen_US
dc.subjectBody weighten_US
dc.subjectEstrous cycleen_US
dc.subjectFemaleen_US
dc.subjectMiceen_US
dc.subjectModels, biologicalen_US
dc.subjectMyometriumen_US
dc.subjectOrgan sizeen_US
dc.subjectPregnancyen_US
dc.subjectPregnancy proteinsen_US
dc.subjectPregnancy, animalen_US
dc.subjectUterusen_US
dc.subjectMedicinal and biomolecular chemistryen_US
dc.subjectBiochemistry and cell biologyen_US
dc.subjectClinical sciencesen_US
dc.subjectBiochemistry & molecular biologyen_US
dc.titleMyometrial mechanoadaptation during pregnancy: implications for smooth muscle plasticity and remodellingen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1111/j.1582-4934.2008.00306.x
pubs.elements-sourceweb-of-scienceen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent Collegeen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent College, Health Sciencesen_US
pubs.publication-statusPublisheden_US


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