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dc.contributor.authorBeermann, Mary Louen_US
dc.contributor.authorArdelt, Magdalenaen_US
dc.contributor.authorGirgenrath, Mahaswetaen_US
dc.contributor.authorMiller, Jeffrey Booneen_US
dc.date.accessioned2012-01-11T22:26:45Z
dc.date.available2012-01-11T22:26:45Z
dc.date.issued2010-4-1
dc.identifier.citationBeermann, Mary Lou, Magdalena Ardelt, Mahasweta Girgenrath, Jeffrey Boone Miller. "Prdm1 (Blimp-1) and the Expression of Fast and Slow Myosin Heavy Chain Isoforms during Avian Myogenesis In Vitro" PLoS ONE 5(4): e9951. (2010)
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2144/3296
dc.description.abstractBACKGROUND. Multiple types of fast and slow skeletal muscle fibers form during early embryogenesis in vertebrates. In zebrafish, formation of the earliest slow myofibers in fin muscles requires expression of the zinc-finger transcriptional repressor Prdm1 (also known as Blimp1). To further understand how the role of Prdm1 in early myogenesis may vary through evolution and during development, we have now analyzed Prdm1 expression in the diverse types of myotubes that form in culture from somitic, embryonic, and fetal chicken myoblasts. PRINCIPAL FINDINGS. In cultures of somitic, embryonic limb, and fetal limb chicken cells, we found that Prdm1 was expressed in all of the differentiated muscle cells that formed, including those that expressed only fast myosin heavy chain isoforms, as well as those that co-expressed both fast and slow myosin heavy chain isoforms. Prdm1 was also expressed in Pax7-positive myoblasts, as well as in non-myogenic cells in the cultures. Furthermore, though all differentiated cells in control somite cultures co-expressed fast and slow myosin heavy chains, antisense knockdown of Prdm1 expression inhibited the formation of these co-expressing cells in somite cultures. CONCLUSIONS. In chicken myogenic cell cultures, Prdm1 was expressed in most Pax7-positive myoblasts and in all differentiated muscle cells, irrespective of the developmental stage of cell donor or the pattern of fast and slow myosin heavy chains expressed in the differentiated cells that were formed. Thus, Prdm1 was expressed in myogenic cells prior to terminal differentiation; and, after differentiation, Prdm1 expression was not limited to cells that expressed slow myosin heavy chain isoforms. In addition, Prdm1 appeared to be required for differentiation of the somitic myocytes, which are the earliest myocytes to form in the avian embryo.en_US
dc.description.sponsorshipNational Research Initiative of the United States Department of Agriculture Cooperative State Research, Education, and Extension Service (#2006-35206-16622); National Heart, Lung, and Blood Institute (2R01HL064641)en_US
dc.language.isoen
dc.publisherPublic Library of Scienceen_US
dc.rightsBeermann et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titlePrdm1 (Blimp-1) and the Expression of Fast and Slow Myosin Heavy Chain Isoforms during Avian Myogenesis In Vitroen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0009951
dc.identifier.pmid20376350
dc.identifier.pmcid2848592


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