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dc.contributor.authorSanborn, Barbara Mortensenen_US
dc.date.accessioned2019-04-08T17:42:35Z
dc.date.issued1965
dc.date.submitted1965
dc.identifier.otherb14569930
dc.identifier.urihttps://hdl.handle.net/2144/34687
dc.descriptionThesis (M.A.)--Boston Universityen_US
dc.descriptionPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.en_US
dc.description.abstractEnzymes are proteins which have the ability to catalyze chemical reactions and which do so with a high degree of specificity. Since trypsin has a sharp specificity for two positively charged substrates, arginine and lysine, an attempt was made to assess the role of the positive charge in determining this specificity. Both Pressman and Wilson did similar studies (Pressman on hapten-antibody reactions and Wilson on acetylcholinesterase reactions) in which they compared the hydrolysis of a substrate possessing a charged quaternary ammonium group with that of the same substrate in which the charged group had been replaced by a tertiary butyl group. In both cases, Ko/K+ was approximately eight where Ko is the dissociation constant for the uncharged substrate and K+ is the dissociation constant for the charged species. The free energy change associated with this change in K is about 1.5 kilocalories per mole and was attributed by both authors to the electrostatic contribution to the binding energy. Attempts to relate these changes in free energy to physical force expressions are discussed. Webb attempted to calculate group separation distances from the free energy changes using the expression for the potential energy of two separated charges corrected for ion atmosphere interactions. He tentatively concluded that for both hapten-antibody and cholinesterase-substrate interactions the groups involved are in direct contact without an intervening water molecule. [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.subjectChemistryen_US
dc.subjectTrypsinen_US
dc.subjectSpecificityen_US
dc.titleThe role of the positive charge in trypsin specificityen_US
dc.typeThesis/Dissertationen_US
dc.description.embargo2031-01-01
etd.degree.nameMaster of Artsen_US
etd.degree.levelmastersen_US
etd.degree.disciplineChemistryen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.barcode11719025585714
dc.identifier.mmsid99181604480001161


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