Show simple item record

dc.contributor.authorColon-Deglans, Jose Oscaren_US
dc.date.accessioned2019-05-16T13:27:02Z
dc.date.available2019-05-16T13:27:02Z
dc.date.issued1994
dc.date.submitted1994
dc.identifier.other(OCoLC)38558761
dc.identifier.otherb21870287
dc.identifier.urihttps://hdl.handle.net/2144/35607
dc.descriptionThesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1994 (Oral Biology).en_US
dc.descriptionIncludes bibliographical references (leaves 57-84).en_US
dc.description.abstractPrevious studies with oral bacterial and fungal pathogens have demonstrated that histatin 5 is the most potent histatin, a group of histidine-rich salivary proteins present in parotid secretions with respect to antibacterial and antifungal effects. The present study was aimed at investigating the ef feet of human parotid salivary histatin? on the viability of the periodontal pathogen Porphyromonas gingivalis (P.g.). Experimentally, the viability of P.g. was studied after exposure to incremental doses of histatin 5 at different pH values (pH 5.0, 6.0, 7.0 and 8.0). Histatin 5 from human parotid secretion was isolated by Bio-Gel P-2 chromatography and reverse-phase high-performance liquid chromatography. The purity of histatin 5 was determined by polyacrylamide gel electrophoresis and amino acid analysis. Following incubation, bacteria were washed by successive centrifugation, plated onto enriched Trypticase soy agar, and incubated anaerobically at 37 °C. After 48 hr, colony forming units were counted. Linear regression analysis was used to evaluate doseresponse. The most perfect fit for a linear regression was found for pH 6.0 and 7.0 (p<0.02) with corresponding LD50 values of 0.19 mM and 0.51 mM, respectively. The data indicate that histatin 5 is bactericidal on P.gingivalis and this effect is modulated by the pH values of the bacterial environment in contact with histatin 5. The relative bactericidal potency was higher at pH 5.0 and decreased as pH increased.en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.en_US
dc.subjectSalivary proteins and peptidesen_US
dc.subjectPorphyromonas gingivalisen_US
dc.subjectOral biologyen_US
dc.titleThe bactericidal effects of human salivary histatin 5 on Porphyromonas gingivalisen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Science in Dentistryen_US
etd.degree.levelmastersen_US
etd.degree.disciplineOral Biologyen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.mmsid


This item appears in the following Collection(s)

Show simple item record