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dc.contributor.authorKandru, Suneel C.en_US
dc.date.accessioned2019-05-16T13:27:35Z
dc.date.available2019-05-16T13:27:35Z
dc.date.issued2007
dc.date.submitted2007
dc.identifier.other(OCoLC)243862973
dc.identifier.other(OCoLC)243862973
dc.identifier.otherb27908586
dc.identifier.urihttps://hdl.handle.net/2144/35615
dc.descriptionThesis (MSD)--Boston University, Goldman School of Dental Medicine, 2007 (Dept. of Endodontics).en_US
dc.descriptionIncludes bibliography: leaves 63-73.en_US
dc.description.abstractThe purpose of the current research was to determine if diabetes had a significant effect on the healing process of an injury caused by bacteria, and to identify the potential mechanisms involved. To achieve the objective of the study, diabetic mice and normoglycemic mice (n=6) were inoculated with Porphyromonas gingivalis (P. gingivalis). Scalp tissue was excised eight days later, and either fixed for histological sections or snap frozen for molecular analysis. Histological analysis from the inoculated site revealed that diabetes increased apoptosis of fibroblast and bone lining cells when compared to normoglycemic animals (p<0.05; n=6). When the pancaspase inhibitor z-V AD-fink (Nbenzyloxycarbonyl- Val-Ala-Asp-fluoromethylketone) was used to inhibit diabetes-enhanced apoptosis, several parameters of healing, including fibroblast density and ir.tcreased matrix formation were observed. Total RNA from the excised scalp tissue obtained from normoglycemic (n=6) and diabetic (n=6) was subjected to RNA profiling, using a Mouse Genome 430 2.0 Array to determine how diabetes affects the global expression of various genes involved in wound healing. The microarray analysis revealed that out of 221 differentially expressed apoptotic genes; diabetes caused more than a twofold induction of 71 genes that directly or indirectly regulate apoptosis. Most of the healing, angiogenesis, growth factor, matrix and immune response genes are highly expressed at 5 days but there is only a moderate increase at 8 days signifying that early defense and wound healing process initiated at 5 days and almost completed at 8 days. Therefore, it was inferred that day 5 is important to study wound healing. And also diabetes-enhanced apoptosis is an important mechanism ... [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.en_US
dc.subjectApoptosisen_US
dc.subjectComputational biologyen_US
dc.subjectDiabetes complicationsen_US
dc.subjectMicroarray analysisen_US
dc.subjectRNA, messengeren_US
dc.subjectWound healingen_US
dc.subjectEndodonticsen_US
dc.titleA bioinformatic analysis of microarray data on the effects of diabetes on increasing mRNA levels of pro-apoptotic genes that contribute to impaired healingen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Science in Dentistryen_US
etd.degree.levelmastersen_US
etd.degree.disciplineEndodonticsen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.mmsid


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