The development of a new bioassay system to assess the ability of drugs to accelerate tooth eruption
Stout, Frank P.
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The purpose of this study was to design a bioassay procedure by means of which it would be possible to rapidly screen drugs to determine their ability to accelerate tooth eruption as well as their undesirable side effects. The rat maxillary 3rd molar was utilized as the assay parameter for measuring acceleration in tooth eruption. The method developed utilizes female rats (Charles River Breeding Lab), 26 days of age. Test drugs are injected twice; when the rats are 26 and 27 days of age; and the rats are sacrificed at 30 days of age. Heads are removed and fixed in 95% ethernol for 12 hours. The upper dental arches are examined under a disecting microscope (lOOX) and the extent of eruption scored. The extent of tooth eruption was determined by comparing 3rd molars to a standardized scoring chart. The scoring chart depicts eleven progressive stages in the eruption of the maxillary right and left 3rd molars. This chart also contains a number or grade for each of the eleven stages (from O, for no eruption, to 10 for full eruption). In control rats there is little or no eruption of the 3rd molars at 30 days of age. Third molar eruption was enhanced more than two times with a 300 ug/kg dose ... [TRUNCATED]
Thesis (M.Sc.D.)--Boston University. Henry M. Goldman School of Graduate Dentistry, 1970 (Orthodontics)
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