Characterization of a novel scaffold for tissue engineering
Tang, Alan Thanh
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Bone regeneration using degradable scaffolds gives clinicians an alternative approach to the repair of damaged tissue while avoiding the need for a permanent implant. The composition of the scaffold material is vital to the success of bone regeneration. The scaffold material used should display biocompatibility, degradability, mechanical integrity, and osteoconductivity. Scaffolds used in this study were made by the melt molding/particulate leaching technique . The synthetic polymer, poly(DL-lactic-coglycolic acid) (PLGA) was used in this study to construct the three dimensional porous scaffolds. This provided the mechanical integrity for scaffold. Bioactive inorganic element (BIE) was incorporated into the scaffold to promote osteogenesis. NaCl was used as a water soluble porogen to create porosities. The objective of this study was to characterize the mechanical strength and solubility of scaffolds with different compositions of PLGNBIE with varying porosities. Scaffolds were made into cylinders 5mm in diameter by 5mm in height. Compressive strength of the scaffolds was tested using an Instron Universal Testing Machine using the femur bone of rabbits with similar dimensions as controls. Solubility of silicon was tested by electron dispersed x-ray anlysis of scaffolds and Fourier Transforming Infrared Spectroscopy of culture medium used in the incubation of the scaffolds at 37°C. Results of compressive strength test indicate that scaffolds with at least 60% PLGA by weight and with no more than 50% porosity has comparable compressive strength to rabbit bone. Scaffolds with more BIE released more silicon as measured by SiOH bond compared to scaffolds with less BIE. Scaffolds with 50% porosity and 50% bioactive inorganic element showed up to 56.6 mg of silicon released into culture medium by 28 days.
Thesis (MSD)--Boston University, Goldman School of Dental Medicine, 2006 (Dept. of Biomaterials).Includes bibliography: leaves 93-100.
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