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dc.contributor.authorXu, Wanpengen_US
dc.date.accessioned2019-05-16T13:35:42Z
dc.date.available2019-05-16T13:35:42Z
dc.date.issued2013
dc.date.submitted2013
dc.identifier.urihttps://hdl.handle.net/2144/35650
dc.descriptionThesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2013 (Department of Periodontology and Oral Biology).en_US
dc.descriptionIncludes bibliographic references: leaves 50-67.en_US
dc.description.abstractObjective: Lysyl oxidase (LOX) dependent cross-linking is deficient in diabetic bone, contributing to osteopenia. Here we Investigate regulation of lysyl oxidase by Wnt3a and TNF-a in a mesenchymal stem cell line as a model for a novel mechanism of diabetic osteopenia. Methods: Wnt3a-treated C3Hl 0Tl/2 cells were treated with or without of TNF-a. Lysyl oxidase expression ( qPCR) and mRNA stability were measured. A comprehensive PCR-based microarray screen (Qiagen/SA Biosciences) was next performed to_ identify miRNAs which regulate LOX mRNA stability in response to TNF-a. The functionality of identified miRNA was independently determined by the use of miRNA mimics. Results: Wnt3a up-regulated LOX up to 3.2-fold by treating C3H10Tl/2 cells with Wnt3a compared to controls. TNF-a (20 ng/ml) blocked Wnt3a up-regulation of LOX. TNF-a decreased the stability of LOX mRNA determined in the presence of a transcription inhibitor in control- and Wnt3a plus TNF-a-treated cells. A micro (miR) array screen performed on RNA isolated from Wnt3a plus TNF-a treated and control cells identified miR203 as being up-regulated 13-fold by TNF-a. Independent analyses ... [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.en_US
dc.subjectDiabetes complicationsen_US
dc.subjectMesenchymal stromal cellsen_US
dc.subjectBone diseases, metabolicen_US
dc.subjectMicroRNAsen_US
dc.subjectTumor necrosis factor-alphaen_US
dc.subjectWnt3a proteinen_US
dc.subjectProtein-lysine 6-oxidaseen_US
dc.titleEffects of TNF-a on lysyl oxidase in Wnt induced cells at post-transcriptional level by miRNAen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Science in Dentistryen_US
etd.degree.levelmastersen_US
etd.degree.disciplinePeriodontology and Oral Biologyen_US
etd.degree.grantorBoston Universityen_US


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