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dc.contributor.advisorDennis, Allisonen_US
dc.contributor.authorNguyen, Thuy Thi Haen_US
dc.date.accessioned2019-06-20T18:16:47Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/2144/36021
dc.description.abstractAn alternative molecular recognition approach was developed for sensing small molecule analytes using the differential binding of an allosteric transcription factor (TF, specifically TetR) to its cognate DNA as the molecular recognition element coupled with Förster resonance energy transfer (FRET) to yield an internally calibrated optical signal transduction mechanism. Sensors were evaluated comprising Cy5-modified DNA (FRET acceptor) with either a tdTomato-TetR fusion protein (FP-TF) or quantum dot-TetR conjugate (QD-TF) as the FRET donor by measuring the ratio of acceptor and donor fluorescence intensities (FA/FD) with titrations of a derivative of the antibiotic tetracycline, anhydrous tetracycline (aTc). A proof-of-concept FRET-based biosensor was successfully demonstrated through the modulation of FA/FD signal intensities based on varying analyte concentrations. Sensor design parameters affecting overall signal-to-noise ratio and sensitivity of the sensors are also identified.en_US
dc.language.isoen_US
dc.rightsAttribution-NonCommercial 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiomedical engineeringen_US
dc.subjectAffinityen_US
dc.subjectAssayen_US
dc.subjectHomogenous assayen_US
dc.subjectNanoparticleen_US
dc.subjectQuantum doten_US
dc.subjectSmall molecule analyteen_US
dc.titleFörster resonance energy transfer (FRET) as an optical readout for transcription factor-DNA binding in biosensing applicationsen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2019-06-04T01:05:13Z
dc.description.embargo2020-06-03T00:00:00Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineBiomedical Engineeringen_US
etd.degree.grantorBoston Universityen_US


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Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial 4.0 International