Microfluidic device for rapid detection of fibrinolysis of blood clots
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This thesis presents a method to detect fibrin formation based on electrical impedance sensing. A polydimethylsiloxane (PDMS) microfluidic device was designed and fabricated to detect fibrin formation in artificial blood samples (solution of fibrinogen, thrombin and CaCl2) by measuring changes in electrical impedance during the process of coagulation. The electrical measurements were performed using a lock-in amplifier. The optical properties of the fibrin in the microfluidic device were observed in an inverted microscope to confirm the clot formation. In the study, the dimensions of the microfluidic devices and the sensing electrodes were optimized to improve the sensitivity and to ensure electrical contact between the electrodes and analyte. An equivalent circuit model was developed to fit the change in the electrical signal of the channel accurately and to determine the optimal measurement frequency. The measured electrical impedances exhibited an expected increase with the blood coagulation. In summary, the technique and the device were shown promising for detecting the blood coagulation process.