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dc.contributor.advisorCohen, Herbert T.en_US
dc.contributor.advisorMcKnight, C. Jamesen_US
dc.contributor.authorNiu, Leilien_US
dc.date.accessioned2019-07-17T15:40:50Z
dc.date.available2019-07-17T15:40:50Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/2144/36586
dc.description.abstractRenal cell carcinoma (RCC) is a common and life-threatening cancer. Among RCCS, clear-cell renal cell carcinoma (ccRCC) is the most prevalent type and is highly malignant. ccRCC is initiated by loss of the von Hippel-Lindau tumor suppressor gene VHL. The pVHL protein binds and stabilzes the renal suppressor protein Jade-1 and exerts important growth suppressive activities through Jade-1. Since many solid tumors, like ccRCC, exhibit chromosomal instability and hence have defects in repair of DNA double-strand breaks (DSBs), we sought to determine the roles of Jade-1 and pVHL in these processes to elucidate mechanisms of cancer development. This study focused on the impact of pVHL on Jade-1 localization and expression levels in renal cancer cells by immunofluorescence microscopy and on Jade-1 expression levels by immunoblotting and qPCR when double-strand DNA damage is introduced using doxorubicin, neocarzinostatin or gamma irradiation. Early results suggested but do not yet confirm that there are differences in Jade-1 localization and expression levels in response to DNA DSBs in renal cells and renal cancer cells with versus without wild-type pVHL. More experiments are required to draw conclusions from these early data.en_US
dc.language.isoen_US
dc.subjectOncologyen_US
dc.titleRole of Jade-1 in DNA repair in renal cell carcinomaen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2019-06-13T01:03:28Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineMedical Sciencesen_US
etd.degree.grantorBoston Universityen_US


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