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dc.contributor.authorCollins, John H.en_US
dc.date.accessioned2019-08-01T16:54:28Z
dc.date.issued1970
dc.date.submitted1970
dc.identifier.otherb14746220
dc.identifier.urihttps://hdl.handle.net/2144/36791
dc.descriptionThesis (Ph.D.)--Boston Universityen_US
dc.descriptionPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.en_US
dc.description.abstractA carbohydrate-free peptide was isolated from a tryptic digest of trifluoroacetylated alpha1-acid glycoprotein (AG) in a yield of 0.9 moles per 44,000 gms. of protein. The purity was established by electrophoresis and chromatography, and the amino acid sequence determined to be: (-Tfa-Lys-Gln-Glu-Glu-Gly-Glu-Ser. The high yield indicated that it was a primary tryptic peptide, yet it did not possess a C-terminal arginine. Since it had been previously shown that AG has serine as its C-terminal residue, it is proposed that the peptide represents the C-terminus of the protein. During the course of this work, more than 100 amino acid analyses were required. An automated ion-exchange type analyzer was not available, and an alternative procedure had to be devised. A system combining quantitative high-voltage electrophoresis and gas-liquid chromatography was standardized and used for almost all of the required analyses. The system proved to be satisfactory for the peptides that were isolated. [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.subjectBiochemistryen_US
dc.subjectGlycoproteinen_US
dc.subjectOrosomucoiden_US
dc.titleStructural studies on alpha1-acid glycoproteinen_US
dc.typeThesis/Dissertationen_US
dc.description.embargo2031-01-01
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineBiochemistryen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.barcode11719025562432
dc.identifier.mmsid99184534970001161


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