Isolation of antibodies to outer membrane proteins from nontypable Haemophilus influenza
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Outer membrane proteins from unencapsulated(nontypable) Haemophilus influenza were prepared by incubation with EDTA and shearing followed by molecular sieve chromatography in a lipooligosaccharide (LOS) disaggregating deoxycholate buffer. Outer membranes proteins collected were determined to be free of LOS by polyacrylamide gel electrophoresis and then covalently attached to cyanogen bromide activated Sepharose. Antibodies to outer membrane proteins were then isolated by affinity chromatography and characterized by enzyme-linked immunosorbant assay. These antibodies will prove useful for passive immunization experiments to establish the role of outer membrane proteins in host protection from infection with nontypable Haemophilus influenza.
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