A platform for brain-wide imaging and reconstruction of individual neurons

Date Issued
2016-01-20Publisher Version
10.7554/eLife.10566Author(s)
Economo, Michael N.
Clack, Nathan G.
Lavis, Luke D.
Gerfen, Charles R.
Svoboda, Karel
Myers, Eugene W.
Chandrashekar, Jayaram
Metadata
Show full item recordPermanent Link
https://hdl.handle.net/2144/37639Version
Published version
Citation (published version)
Michael N Economo, Nathan G Clack, Luke D Lavis, Charles R Gerfen, Karel Svoboda, Eugene W Myers, Jayaram Chandrashekar. 2016. "A platform for brain-wide imaging and reconstruction of individual neurons.." Elife, Volume 5:e10566. https://doi.org/10.7554/eLife.10566Abstract
The structure of axonal arbors controls how signals from individual neurons are routed within the mammalian brain. However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue. We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data. We demonstrate the power of this approach by reconstructing the axonal arbors of multiple neurons in the motor cortex across a single mouse brain.
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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.Collections