A platform for brain-wide imaging and reconstruction of individual neurons
Economo, Michael N.
Clack, Nathan G.
Lavis, Luke D.
Gerfen, Charles R.
Myers, Eugene W.
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Citation (published version)Michael N Economo, Nathan G Clack, Luke D Lavis, Charles R Gerfen, Karel Svoboda, Eugene W Myers, Jayaram Chandrashekar. 2016. "A platform for brain-wide imaging and reconstruction of individual neurons.." Elife, Volume 5:e10566. https://doi.org/10.7554/eLife.10566
The structure of axonal arbors controls how signals from individual neurons are routed within the mammalian brain. However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue. We introduce a platform for high-resolution, three-dimensional fluorescence imaging of complete tissue volumes that enables the visualization and reconstruction of long-range axonal arbors. This platform relies on a high-speed two-photon microscope integrated with a tissue vibratome and a suite of computational tools for large-scale image data. We demonstrate the power of this approach by reconstructing the axonal arbors of multiple neurons in the motor cortex across a single mouse brain.
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