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dc.contributor.advisorSegrè, Danielen_US
dc.contributor.authorJo, Charlesen_US
dc.date.accessioned2019-09-24T15:11:36Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/2144/37987
dc.description.abstractThis thesis reports the development of BioMe, a co-culture microplate platform that enables high-throughput, real-time quantitative growth dynamics measurements of interacting microbial batch cultures. The primary BioMe components can be 3D-printed, allowing ease of fabrication and DIY accessibility in the microbiome community. A pairwise 3D-printed iteration of the BioMe device was used in diffusion and co-culture experiments. Genetically engineered Escherichia Coli lysine and isoleucine auxotroph strains were used to characterize the diffusion of amino acids across the porous membranes. Results demonstrated a nonlinear relationship between growth rate and pore size and also distinct diffusion behavior for lysine and isoleucine. Pairwise syntrophic co-culture experiments demonstrated synergistic but repressed interaction between these two paired auxotrophs. Investigation of the effect of varying initial amino acid conditions on growth dynamics demonstrated that small changes in initial media condition can consistently affect patterns of yield and growth rate of constituent microbial species.en_US
dc.language.isoen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectBiomedical engineeringen_US
dc.subject3D-printingen_US
dc.subjectMicrobiomeen_US
dc.subjectSynthetic ecologyen_US
dc.titleA co-culture microplate platform to quantify microbial interactions and growth dynamicsen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2019-08-30T19:04:18Z
dc.description.embargo2020-08-30T00:00:00Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineBiomedical Engineeringen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.orcid0000-0001-8313-6234


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Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International