The role of PDGF-A-fibronectin interactions in the directed migration of the mesendoderm during gastrulation
Smith, Erin M.
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Platelet-derived growth factor-A (PDGF-A) is essential for processes involving cell motility throughout embryonic development. Here we examined the underlying basis of its role as a guidance cue for the directed migration of anterior mesendoderm cells during a period of cell reorganizations called gastrulation. In Xenopus laevis embryos, these cells migrate across a fibronectinrich extracellular matrix (ECM) that lines the blastocoel roof (BCR). We tested the hypothesis that PDGF-A mRNA is in a graded pattern across the BCR that is modulated by mesendoderm cells as they move across it. Quantitative reverse transcriptase polymerase chain reactions were performed on BCR tissue sections. Throughout gastrulation, the overall levels of PDGF-A mRNA increased, and at all developmental stages examined, the same pattern of PDGF-A mRNA was identified. PDGF-A mRNA levels were lowest at the blastopore lip and although higher levels were detected across the rest of the ... [TRUNCATED]
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