Pro-resolving lipid mediators (RvE1, RvD1, LXA4 & 15-epi-LXA4) and platelet rich plasma accelerate the osteoblastic and fibroblastic activity in wound healing process
Al-Qatami, Fawzi M.
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Objectives: The aim of this study was to investigate the impact of pro-resolution lipid mediators (RvE1 , RvD1 , LXA4 & 15-epi-LX A4) and platelet rich plasma on osteoblasts and fibroblasts. Materials & Methods: A cellular wound injury model was designed for two osteoblastic cell lines (Saos-2 and MC3T3-E1) and a fibroblastic cell line (primary gingival fibroblasts). RvE1, RvD1, LXA4 & 1 5-epi-LX A4 and platelet rich plasma (PRP) were added to test the impact on wound cIosure rate. Alkaline phosphatase activity was measured for osteoblastic function. Osteocalcin levels were measured for the osteoblast activity. Fibronectin production was measured as a function of fibroblasts. Results: Wound closure rates in all three cell lines were increased significantly when PRP alone was added. Lipid mediators significantly enhanced the wound cIosure rate. There was a parallel increase in alkaline phosphatase and osteocalcin activity in osteoblastic activity. The fibronectin level was the highest in the PRP & RvD1 group on day 7. Conclusion: Lipid mediator, which resolve the inflammation, actively enhance the PRP-mediated wound healing process by the osteoblasts and fibroblasts.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2011 (Department of Orthodontics and Dentofacial Orthopedics).Includes bibliography: leaves 103-114.
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