The validity of RANKL as a salivary marker for condylar osteoarthritis
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Objective: To test the validity of RANKL as a marker for condylar osteoarthritis (OA) when collected from saliva. Design: 20 subjects with OA and 20 healthy subjects were recruited. All subject (32 females and 8 males, age ranged from 12 to 38 years (mean 34 years/ SD 8.4)) had CBCT taken. OA scans were classified according to severity on sagittal and coronal slices. Salivary markers of RANKL and OPG were primarily analyzed using a Multiplex Immunoassay(Luminex). In addition, salivary levels of proinflammatory markers including IL-1[alpha], IL-1[beta], TNF-[alpha], IL-6, GM-CSF, MIP-1[alpha], VEGF, and TGF-[beta]1, and matrix metalloproteinases MMP-3, -8, -9 were analyzed. Correlations between levels of these markers with the degree of tissue destruction induced were computed. Association of extent of bone destruction to the clinical symptom was examined. Results: No significant difference was found between levels of RANKL and OPG in healthy and OA subject. No significant correlations were established between levels of these markers with degree of bone destruction, or extent of bone destruction with the clinical symptom. Statistically significant differences were found in the salivary levels of TNF-[alpha], MMP-3 and GM-CSF. Conclusion: Based on this study results, salivary RANKL and OPG are invalid makers for condylar osteoarthritis. However, previously reported proinflammatory markers and matrix metalloproteinases are significant indicators of the condition in saliva samples. Saliva can be an important tool to detect inflammatory changes due to condylar osteoarthritis.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (DScD) --Boston University, Henry M. Goldman School of Dental Medicine, 2015 (Department of Orthodontics and Dentofacial Orthopedics).Includes bibliographic references: leaves 55-65.
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