The effect of salivary proteins on wound healing
Alnajdi, Maryam M.
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Oral wounds heal faster with reduced levels of inflammation as well as scaring and saliva is thought to play a major role in this property. One of theearly steps in wound healing is re-epithelialization, which takes place by the migration of epithelial cells at the wound edges. The phenotypic changes that enable differentiated epithelial cells to migrate are caused by signals resulting from interaction with growth factors as well as other extra-cellular matrix proteins. While most studies on oral wound healing have focused on salivary growth factors, recent findings have implicated salivary histatins as potential players in this process. In our study, we tested the role of saliva and salivary histatins in enhancing wound healing, by sfudying their effects on a buccal epithelial cells line. WSS, PS and synthetic salivary histatins (namely Histatins 1, 3 and 5) were used in the scratch assay and in the Boyden chamber assay, two widely used assays for cell migration. We also tested the effects of a degradation product of histatin 1, which is histatin 1 27/38. migration using the scratch assay, but not the Boyden chamber assay. The fragment histatin 1 27/38 was found to be inactive using the assay but showed stimulation in the Boyden chamber assay. Histatin 5 did not stimulate cell migration. These data add to the already established anti-fungal roles of salivary histatins, demonstrating the ability of some histatins as potentially novel wound healing agents.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2011 (Department of Periodontology and Oral Biology).Includes bibliography: leaves 50-59.
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