The osteogenic effects of mineral trioxide aggregate and modified mineral trioxide aggregate on normal human osteoblast cells in vitro
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The aim of this study was to investigate, on normal human osteoblast cells, the osteogenic effects of a synthetic Mineral Trioxide Aggregate (SMTA), SMTA+20% silicon, SMTA+40% silicon, SMTA+20% bioactive inorganic element (BIE) and SMTA+40% BIE. The materials were mixed and incubated 24 hours prior to testing in 24 wells plates. Osteoblasts were obtained from culturing bone chips from eight healthy patients, then seeded at a density of 1.5×10 cells on each well. Cells grown alone on the wells served as a control. Crystal violet dye was used to measure cell attachment efficiency and proliferation rate at 16h, 12 and 20 days. SEM examination was done at 12 days, using the SMTA and SMTA-Silicon 40% samples, with and without cells. Osteocalcin levels were measured by immunoradiometric assay at 12 and 20 days. The data were statistically analyzed with an analysis of variance (ANOVA) and post-hoc testing compared means by treatment group. The overall proliferation results showed that both the SMTA-BIE40% and the SMTA-Si40% groups had a higher cell attachment efficiency compared to the SMTA and the control groups (P[less than]0.05). The control group had a higher level of osteocalcin than the SMTA-BIE20%, SMTA-Si20% and SMTA-Si40% (p[less than]0.05). There was no significant difference in osteocalcin levels between the control group and the SMTA and SMTA-BIE40% groups. The SEM examination showed cell attachment on both SMTA and SMTA-Si40% samples. As a conclusion, modified MTA with addition of 40% BIE could promote the proliferation of normal human osteoblast cells in vitro. Supported by BU Department of Biomaterials.
Thesis (MSD)--Boston University, Goldman School of Dental Medicine, 2008 (Dept. of Restorative Sciences/Biomaterials).Includes bibliographical references: leaves 110-123.
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