Internal geometry of scaffold and its effect on cell seeding
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One of the recent applications of absorbable biomaterials is in the growing field of tissue engineering. The objective of this study is to study the effect of the internal geometry of the porous scaffolds on the cell distribution. The three-dimensional distribution of cells greatly affects the outcome of tissue engineering. A basic requirement involves a scaffold conductive to cell seeding and maintenance of cell function. To design scaffolds in this study, synthetic polymer, PLGA, was used to provide mechanical strength, Bioactive Inorganic Element (BIE) was included to stimulate osteogenesis and salt (NaCI) was used to create pores. Two groups of channeled and non-channeled porous scaffolds of PLGA/BIE, 10 mm in width and 12 mm in length, were fabricated by melt molding/particulate leaching. In channeled scaffold 6 peripheral channels and one central channel was placed. Each peripheral channel was placed 2.5 mm from the border of the scaffold, and 2.5 mm from the central channel, and 2 mm from the adjacent channel. Normal human osteoblasts were extracted from the alveolar bone of young healthy adults, and expanded to the second passage. Cells were cultured, stained with Neutral Red, and seeded onto scaffold at a concentration of 1.5x10 cells/cm. Scaffolds were fixed with formaldehyde, paraffin molded and sectioned. Sections were studied under the light microscope to evaluate the depth of the cell distribution. Results revealed that at p-value of 7x10[-8] there was more depth of distribution of bone cells in channeled scaffolds compared to the control group.
Thesis(M.S.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Endodontics).Includes bibliographical references (leaves 98-108).
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