Detection of mycoplasma fermentans in HIV seropositive individuals
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In an attempt to determine the prevalence of Mycoplasma fermentos in patients with human immunodeficiency virus infection, a polymerase chain-based assay was used to detect M.fermentans DNA from whole saliva. This assay selectively amplifies a 206-nuCleotide gene sequence within an insertion sequence-like element unique to Mycoplasma fermentans. The anplication product was detected in Ethidium bromide stained 6% polyacrylamide gel and confirmed by southern blotting with a probe base on an internal sequence of the expected amplification product. M fermentans-specific amplification products were found in saliva from 18% (4) of 22 HIV-seropositive with CD4 superscript +[more than]200 samples, 35% (1 1) of 32 HIV-seropositive with CD4 superscript + [less than]200 samples and 31% (9) of 29 HIV seronegative samples. The data suggest that M.fermentants is often found in human saliva and that there is no association in prevalence of this organism among each group of samples. These results must be confirmed by other investigators, and further studies should be undertaken to determine the clinical significance of M. /fermentans infection.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1998 (Pedodontics).
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