Immunolocalization of bone matrix macromolecules in tissues regenerated in periodontal defects treated with expanded plytetrafluoroethylene membranes
Farhat, Fouad N.
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Guided tissue regeneration is a technique that allows the repopulation of periodontal wounds by specific cells, resulting in the reconstitution of a new attachment apparatus. The biologic mechanisms and the sequence of events that occur under the barrier membrane that lead to regeneration are still not completely understood. To help understand this cascade of events, specific matrix macromolecules believed to be phenotypic of bone and cementum formation in tissues grown under the GTR barrier were localized by means of immunohistochemistry. Periodontal regeneration was initiated by placing membranes in experimentally induced periodontal defects in a rhesus monkey model. Samples were harvested 6 weeks after healing and sections of soft tissues grown under GTR barriers (membrane tissue) were stained with antibodies to Bone Morphogenetic Protein -2 and 7 (BMP-2 and BMP-7), as well as antibody for type XII collagen. Tissues grown in the absence of any barrier device served as control (control tissue). The membrane tissue sections showed spindle-shaped fibroblast like cells encased in a dense fibrillar extracellular matrix. Round shaped cells aggregated to form nodules. A similar but very disorganized, fiber network was observed in the control tissues, but neither nodules nor hard tissue formation was observed. Immunohistochemical staining showed very specific stain for BMP-2, BMP-7 and Collagen XII in the membrane tissues as compared to a much weaker and less specific faint stain in the control tissues. When the profile of BMP-7, BMP-2 and Collagen type XII was analyzed on membrane tissue sections, striking similarities were noted in the connective tissue adjacent to the newly formed hard tissue and in nodular areas. The correlation between these histochemical findings strongly suggests that the newly regenerated soft tissue under ePTFE membranes contain cells and extracellular matrix macromolecules normally associated with bone and cementum.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Oral Biology).Includes bibliographical references (leaves 53-67).
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