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dc.contributor.authorBoassa, Danielaen_US
dc.contributor.authorLemieux, Sakina P.en_US
dc.contributor.authorLev-Ram, Vardaen_US
dc.contributor.authorHu, Junruen_US
dc.contributor.authorXiong, Qingen_US
dc.contributor.authorPhan, Sebastianen_US
dc.contributor.authorMackey, Masonen_US
dc.contributor.authorRamachandra, Ranjanen_US
dc.contributor.authorPeace, Ryan E.en_US
dc.contributor.authorAdams, Stephen R.en_US
dc.contributor.authorEllisman, Mark H.en_US
dc.contributor.authorNgo, John T.en_US
dc.date2019-05-13
dc.date.accessioned2020-01-09T19:30:29Z
dc.date.available2020-01-09T19:30:29Z
dc.date.issued2019-10-17
dc.identifier.citationDaniela Boassa, Sakina Lemieux, Varda Lev-Ram, Junru Hu, Qing Xiong, Sebastian Phan, Mason Mackey, Ranjan Ramachandra, Ryan Peace, Stephen Adams, Mark Ellisman, and John T. Ngo. 2019. "Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy." Cell Chemical Biology, Volume 26, Issue 10, Pages 1407-1416.e5. https://doi.org/10.1016/j.chembiol.2019.07.007
dc.identifier.issn2451-9456
dc.identifier.urihttps://hdl.handle.net/2144/39061
dc.descriptionPlease note: this work is indefinitely embargoed in OpenBU. No public access is forecasted. To request access, please click on the lock icon and fill out the appropriate web form.en_US
dc.description.abstractA protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional reporter that permits tagged protein complexes to be visualized by fluorescence light microscopy (LM), and then by standard as well as “multicolor” electron microscopy (EM) via the photooxidation of 3-3’-diaminobenzidine (DAB) and its derivatives.en_US
dc.publisherElsevieren_US
dc.relation.ispartofCell Chemical Biology
dc.titleSplit-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopyen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1016/j.chembiol.2019.07.007
pubs.elements-sourcemanual-entryen_US
pubs.notesEmbargo: Indefiniteen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0003-3508-1915 (Ngo, John)
dc.identifier.mycv475208


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