Monocyte chemoattractant protein-1 expression and monocyte recruitment in osseous inflammation
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In bone, early events in inflammation involve production and release of primary pro-inflammatory cytokines such as IL-1[Beta]. By activation of target cells, these cytokines are thought to induce a second wave of cytokines including monocyte chemoattractant protein-1 (MCP-1). MCP-1 is a chemokine that specifically stimulates monocytes/macrophages and basophils. Experiments were undertaken to examine the expression of MCP-1 in bone associated cells both in vivo and in vitro. To observe in vivo expression of MCP-1, an inflammatory lesion was created in the murine mandible. Immunohistochemistry experiments utilizing specific antibodies to MCP- 1 were conducted to identify MCP-1 expressing cells in inflamed and non-inflamed bone. We found that osteoblasts were the principal cells expressing MCP-1 in inflamed bone. There was little or no MCP-1 expression in non-inflamed bone. Immunohistochemistry experiments were carried out to assess monocyte recruitment during osseous inflammation. The number of MCP-1 positive cells was significantly correlated to the number of monocytes/macrophages present (N=15, r = 0.69, p is [equal to or less than] 0.01). We next examined the ability of isolated bone cells to produce MCP-1 in response to IL-1[Beta] in vitro. Normal osteoblasts and osteoclasts were collected from murine long bones, and were incubated with IL-1[Beta]. Immunohistochemistry was carried out utilizing specific antibodies to MCP-1. Both osteoblasts and osteoclasts expressed MCP-1 when pre-incubated with IL-1[Beta], but did not express MCP-1 without IL-1[Beta] stimulation. These in vitro and in vivo results strongly suggest that MCP-1 is an important mediator involved in the recruitment of monocytes/macrophages in inflamed bone.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (M.Sc.D.)--Boston University. Henry M. Goldman School of Graduate Dentistry, 1995 (Orthodontics).Includes bibliographic references (leaves 46-57).
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