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dc.contributor.authorRahimi, Pourangen_US
dc.date.accessioned2020-03-04T17:15:27Z
dc.date.available2020-03-04T17:15:27Z
dc.date.issued1995
dc.date.submitted1995
dc.identifier.other(OCoLC)38426340
dc.identifier.otherb21845001
dc.identifier.urihttps://hdl.handle.net/2144/39717
dc.descriptionPLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.descriptionThesis (M.Sc.D.)--Boston University. Henry M. Goldman School of Graduate Dentistry, 1995 (Orthodontics).en_US
dc.descriptionIncludes bibliographic references (leaves 46-57).en_US
dc.description.abstractIn bone, early events in inflammation involve production and release of primary pro-inflammatory cytokines such as IL-1[Beta]. By activation of target cells, these cytokines are thought to induce a second wave of cytokines including monocyte chemoattractant protein-1 (MCP-1). MCP-1 is a chemokine that specifically stimulates monocytes/macrophages and basophils. Experiments were undertaken to examine the expression of MCP-1 in bone associated cells both in vivo and in vitro. To observe in vivo expression of MCP-1, an inflammatory lesion was created in the murine mandible. Immunohistochemistry experiments utilizing specific antibodies to MCP- 1 were conducted to identify MCP-1 expressing cells in inflamed and non-inflamed bone. We found that osteoblasts were the principal cells expressing MCP-1 in inflamed bone. There was little or no MCP-1 expression in non-inflamed bone. Immunohistochemistry experiments were carried out to assess monocyte recruitment during osseous inflammation. The number of MCP-1 positive cells was significantly correlated to the number of monocytes/macrophages present (N=15, r = 0.69, p is [equal to or less than] 0.01). We next examined the ability of isolated bone cells to produce MCP-1 in response to IL-1[Beta] in vitro. Normal osteoblasts and osteoclasts were collected from murine long bones, and were incubated with IL-1[Beta]. Immunohistochemistry was carried out utilizing specific antibodies to MCP-1. Both osteoblasts and osteoclasts expressed MCP-1 when pre-incubated with IL-1[Beta], but did not express MCP-1 without IL-1[Beta] stimulation. These in vitro and in vivo results strongly suggest that MCP-1 is an important mediator involved in the recruitment of monocytes/macrophages in inflamed bone.en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.subjectOsteoblastsen_US
dc.subjectOsteoclastsen_US
dc.subjectCytokinesen_US
dc.subjectChemokinesen_US
dc.titleMonocyte chemoattractant protein-1 expression and monocyte recruitment in osseous inflammationen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Science in Dentistryen_US
etd.degree.levelmastersen_US
etd.degree.disciplineOrthodonticsen_US
etd.degree.grantorBoston Universityen_US


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