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dc.contributor.authorKim, Jong-Heeen_US
dc.contributor.authorGraber, Ted G.en_US
dc.contributor.authorLiu, Haimingen_US
dc.contributor.authorAsakura, Atsushien_US
dc.contributor.authorThompson, LaDora V.en_US
dc.coverage.spatialUnited Statesen_US
dc.date2019-03-26
dc.date.accessioned2020-04-08T15:46:08Z
dc.date.available2020-04-08T15:46:08Z
dc.date.issued2019
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/30964931
dc.identifier.citationJong-Hee Kim, Ted G Graber, Haiming Liu, Atsushi Asakura, LaDora V Thompson. 2019. "Increasing myosin light chain 3f (MLC3f) protects against a decline in contractile velocity.." PLoS One, Volume 14, Issue 4, https://doi.org/10.1371/journal.pone.0214982
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2144/40051
dc.description.abstractDisuse induces adaptations in skeletal muscle, which lead to muscle deterioration. Hindlimb-unloading (HU) is a well-established model to investigate cellular mechanisms responsible for disuse-induced skeletal muscle dysfunction. In myosin heavy chain (MHC) type IIB fibers HU induces a reduction in contraction speed (Vo) and a reduction in the relative myosin light chain 3f (MLC3f) protein content compared with myosin light chain 1f (MLC1f) protein. This study tested the hypothesis that increasing the relative MLC3f protein content via rAd-MLC3f vector delivery would attenuate the HU-induced decline in Vo in single MHC type IIB fibers. Fischer-344 rats were randomly assigned to one of three groups: control, HU for 7 days, and HU for 7 days plus rAd-MLC3f. The semimembranosus muscles were injected with rAd-MLC3f (3.75 x 1011-5 x 1011 ifu/ml) at four days after the initiation of HU. In single MHC type IIB fibers the relative MLC3f content decreased by 25% (12.00±0.60% to 9.06±0.66%) and Vo was reduced by 29% (3.22±0.14fl/s vs. 2.27±0.08fl/s) with HU compared to the control group. The rAd-MLC3f injection resulted in an increase in the relative MLC3f content (12.26±1.19%) and a concomitant increase in Vo (2.90±0.15fl/s) of MHC type IIB fibers. A positive relationship was observed between the percent of MLC3f content and Vo. Maximal isometric force and specific tension were reduced with HU by 49% (741.45±44.24μN to 379.09±23.77μN) and 33% (97.58±4.25kN/m2 to 65.05±2.71kN/m2), respectively compared to the control group. The rAd-MLC3f injection did not change the HU-induced decline in force or specific tension. Collectively, these results indicate that rAd-MLC3f injection rescues hindlimb unloading-induced decline in Vo in MHC type IIB single muscle fibers.en_US
dc.languageeng
dc.language.isoen_US
dc.relation.ispartofPLOS One
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAdaptation, physiologicalen_US
dc.subjectAdenoviridaeen_US
dc.subjectAnimalsen_US
dc.subjectGenetic vectorsen_US
dc.subjectMaleen_US
dc.subjectMuscle contractionen_US
dc.subjectMuscle fibers, skeletalen_US
dc.subjectMuscular disorders, atrophicen_US
dc.subjectTransduction, geneticen_US
dc.subjectRats, inbreden_US
dc.subjectRatsen_US
dc.subjectMyosin light chainsen_US
dc.subjectHindlimb suspensionen_US
dc.subjectGeneral science & technologyen_US
dc.titleIncreasing myosin light chain 3f (MLC3f) protects against a decline in contractile velocityen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1371/journal.pone.0214982
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent Collegeen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent College, Physical Therapy and Athletic Trainingen_US
pubs.publication-statusPublished onlineen_US
dc.identifier.mycv457269


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International