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dc.contributor.authorDaaboul, George G.en_US
dc.contributor.authorGagni, Paolaen_US
dc.contributor.authorBenussi, Luisaen_US
dc.contributor.authorBettotti, Paoloen_US
dc.contributor.authorCiani, Miriamen_US
dc.contributor.authorCretich, Marinaen_US
dc.contributor.authorFreedman, David S.en_US
dc.contributor.authorGhidoni, Robertaen_US
dc.contributor.authorOzkumur, Ayca Yalcinen_US
dc.contributor.authorPiotto, Chiaraen_US
dc.contributor.authorProsperi, Davideen_US
dc.contributor.authorSantini, Benedettaen_US
dc.contributor.authorUnlu, M. Selimen_US
dc.contributor.authorChiari, Marcellaen_US
dc.date.accessioned2020-04-13T13:39:10Z
dc.date.available2020-04-13T13:39:10Z
dc.date.issued2016-11-17
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000388266300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e74115fe3da270499c3d65c9b17d654
dc.identifier.citationGeorge G Daaboul, Paola Gagni, Luisa Benussi, Paolo Bettotti, Miriam Ciani, Marina Cretich, David S Freedman, Roberta Ghidoni, Ayca Yalcin Ozkumur, Chiara Piotto, Davide Prosperi, Benedetta Santini, M Selim Unlu, Marcella Chiari. 2016. "Digital Detection of Exosomes by Interferometric Imaging." SCIENTIFIC REPORTS, Volume 6, 10 pp. https://doi.org/10.1038/srep37246
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2144/40115
dc.description.abstractExosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics. The small size (30–100 nm) along with low refractive index contrast of exosomes makes direct characterization and phenotypical classification very difficult. In this work we present a method based on Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) that allows multiplexed phenotyping and digital counting of various populations of individual exosomes (>50 nm) captured on a microarray-based solid phase chip. We demonstrate these characterization concepts using purified exosomes from a HEK 293 cell culture. As a demonstration of clinical utility, we characterize exosomes directly from human cerebrospinal fluid (hCSF). Our interferometric imaging method could capture, from a very small hCSF volume (20 uL), nanoparticles that have a size compatible with exosomes, using antibodies directed against tetraspanins. With this unprecedented capability, we foresee revolutionary implications in the clinical field with improvements in diagnosis and stratification of patients affected by different disorders.en_US
dc.description.sponsorshipThis work was supported by Regione Lombardia and Fondazione Cariplo through POR-FESR, project MINER (ID 46875467); Italian Ministry of Health, Ricerca Corrente. This work was partially supported by The Scientific and Technological Research Council of Turkey (grant #113E643). (Regione Lombardia; 46875467 - Fondazione Cariplo through POR-FESR, project MINER; Italian Ministry of Health, Ricerca Corrente; 113E643 - Scientific and Technological Research Council of Turkey)en_US
dc.format.extent10 pagesen_US
dc.languageEnglish
dc.language.isoen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.relation.ispartofSCIENTIFIC REPORTS
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0
dc.subjectScience & technologyen_US
dc.subjectNanoparticle tracking analysisen_US
dc.subjectExtracellular vesiclesen_US
dc.subjectLabel-freeen_US
dc.subjectUrinary exosomesen_US
dc.subjectMicroparticlesen_US
dc.subjectBiomarkeren_US
dc.subjectProteinsen_US
dc.subjectDiseaseen_US
dc.subjectPlasmaen_US
dc.subjectCerebrospinal fluiden_US
dc.subjectExosomesen_US
dc.subjectHEK293 cellsen_US
dc.subjectHumansen_US
dc.subjectInterferometryen_US
dc.subjectMicroarray analysisen_US
dc.subjectBiochemistry and cell biologyen_US
dc.titleDigital detection of exosomes by interferometric imagingen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1038/srep37246
pubs.elements-sourceweb-of-scienceen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Electrical & Computer Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0002-8594-892X (Unlu, M Selim)
dc.identifier.mycv120083


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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
Except where otherwise noted, this item's license is described as This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.