Show simple item record

dc.contributor.authorSevenler, Derinen_US
dc.contributor.authorTrueb, Jacoben_US
dc.contributor.authorUnlu, M. Selimen_US
dc.date.accessioned2020-04-13T15:14:49Z
dc.date.available2020-04-13T15:14:49Z
dc.date.issued2019-03-05
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000460242100036&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e74115fe3da270499c3d65c9b17d654
dc.identifier.citationDerin Sevenler, Jacob Trueb, M Selim Unlu. 2019. "Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume 116, Issue 10, pp. 4129 - 4134 (6). https://doi.org/10.1073/pnas.1815329116
dc.identifier.issn0027-8424
dc.identifier.urihttps://hdl.handle.net/2144/40122
dc.description.abstractThe clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout. These endpoint assays are now primarily limited by the affinity and specificity of the molecular-recognition agents for the analyte of interest. In contrast, a kinetic assay with single-molecule readout could distinguish between low-abundance, high-affinity (specific analyte) and high-abundance, low-affinity (nonspecific background) binding by measuring the duration of individual binding events at equilibrium. Here, we describe such a kinetic assay, in which individual binding events are detected and monitored during sample incubation. This method uses plasmonic gold nanorods and interferometric reflectance imaging to detect thousands of individual binding events across a multiplex solid-phase sensor with a large area approaching that of leading bead-based endpoint-assay technologies. A dynamic tracking procedure is used to measure the duration of each event. From this, the total rates of binding and debinding as well as the distribution of binding-event durations are determined. We observe a limit of detection of 19 fM for a proof-of-concept synthetic DNA analyte in a 12-plex assay format.en_US
dc.format.extentp. 4129 - 4134en_US
dc.languageEnglish
dc.language.isoen_US
dc.publisherNATL ACAD SCIENCESen_US
dc.relation.ispartofPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
dc.subjectScience & technologyen_US
dc.subjectSingle-molecule detectionen_US
dc.subjectBiosensoren_US
dc.subjectNanotechnologyen_US
dc.subjectNanophotonicsen_US
dc.subjectMolecular recognitionen_US
dc.subjectCirculating tumor DNAen_US
dc.subjectNucleic-aciden_US
dc.subjectProteinen_US
dc.subjectDiagnosisen_US
dc.subjectNanorodsen_US
dc.subjectBiosensing techniquesen_US
dc.subjectDNAen_US
dc.subjectGolden_US
dc.subjectInterferometryen_US
dc.subjectMetal nanoparticlesen_US
dc.subjectNanotubesen_US
dc.titleBeating the reaction limits of biosensor sensitivity with dynamic tracking of single binding eventsen_US
dc.typeArticleen_US
dc.description.versionFirst author draften_US
dc.identifier.doi10.1073/pnas.1815329116
pubs.elements-sourceweb-of-scienceen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Electrical & Computer Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0002-8594-892X (Unlu, M Selim)
dc.identifier.mycv397258


This item appears in the following Collection(s)

Show simple item record