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    Simultaneous evaluation of multiple microarray surface chemistries through real-time interferometric imaging.

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    Date Issued
    2020-01-04
    Publisher Version
    10.1007/s00216-019-02276-1
    Author(s)
    Chiodi, Elisa
    Sola, Laura
    Brambilla, Dario
    Cretich, Marina
    Marn, Allison Marie
    Ünlü, M. Selim
    Chiari, Marcella
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    Permanent Link
    https://hdl.handle.net/2144/40124
    OA Version
    First author draft
    Citation (published version)
    Elisa Chiodi, Laura Sola, Dario Brambilla, Marina Cretich, Allison Marie Marn, M. Selim Ünlü, Marcella Chiari. 2020. "Simultaneous evaluation of multiple microarray surface chemistries through real-time interferometric imaging.." Analytical Bioanalytical Chemistry, https://doi.org/10.1007/s00216-019-02276-1
    Abstract
    Surface chemistry is a crucial aspect for microarray modality biosensor development. The immobilization capability of the functionalized surface is indeed a limiting factor for the final yield of the binding reaction. In this work, we were able to simultaneously compare the functionality of protein ligands that were locally immobilized on different polymers, while on the same solid support, therefore demonstrating a new way of multiplexing. Our goal was to investigate, in a single experiment, both the immobilization efficiency of a group of reactive polymers and the resulting affinity of the tethered molecules. This idea was demonstrated by spotting many reactive polymers on a Si/SiO2 chip and depositing the molecular probes on the spots immediately after. As a proof of concept, we focused on which polymers would better immobilize a model protein (α-Lactalbumin) and a peptide (LAC-1). We successfully showed that this protocol is applicable to proteins and peptides with a good efficiency. By means of real-time binding measurements performed with the interferometric reflectance imaging sensor (IRIS), local functionalization proved to be comparable to the classical flat coating solution. The final outcome highlights the multiplexing power of this method: first, it allows to characterize dozens of polymers at once. Secondly, it removes the limitation, related to coated surfaces, that only molecules with the same functional groups can be tethered to the same solid support. By applying this protocol, many types of molecules can be studied simultaneously and immobilization for each probe can be individually optimized.
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    • ENG: Electrical and Computer Engineering: Scholarly Papers [376]
    • BU Open Access Articles [4751]


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