Show simple item record

dc.contributor.authorSevenler, Derinen_US
dc.contributor.authorDaaboul, Georgeen_US
dc.contributor.authorEkiz-Kanik, Fulyaen_US
dc.contributor.authorUnlu, M. Selimen_US
dc.date.accessioned2020-04-13T17:25:57Z
dc.date.available2020-04-13T17:25:57Z
dc.date.issued2018-01-23
dc.identifierhttp://arxiv.org/abs/1801.07649v1
dc.identifier.citationDerin Sevenler, George Daaboul, Fulya Ekiz-Kanik, M Selim Unlu. "A digital microarray using interferometric detection of plasmonic nanorod labels."
dc.identifier.urihttps://hdl.handle.net/2144/40130
dc.description.abstractDNA and protein microarrays are a high-throughput technology that allow the simultaneous quantification of tens of thousands of different biomolecular species. The mediocre sensitivity and dynamic range of traditional fluorescence microarrays compared to other techniques have been the technology's Achilles' Heel, and prevented their adoption for many biomedical and clinical diagnostic applications. Previous work to enhance the sensitivity of microarray readout to the single-molecule ('digital') regime have either required signal amplifying chemistry or sacrificed throughput, nixing the platform's primary advantages. Here, we report the development of a digital microarray which extends both the sensitivity and dynamic range of microarrays by about three orders of magnitude. This technique uses functionalized gold nanorods as single-molecule labels and an interferometric scanner which can rapidly enumerate individual nanorods by imaging them with a 10x objective lens. This approach does not require any chemical enhancement such as silver deposition, and scans arrays with a throughput similar to commercial fluorescence devices. By combining single-nanoparticle enumeration and ensemble measurements of spots when the particles are very dense, this system achieves a dynamic range of about one million directly from a single scan.en_US
dc.language.isoen_US
dc.subjectQuantitative biologyen_US
dc.subjectBiomoleculesen_US
dc.titleA digital microarray using interferometric detection of plasmonic nanorod labelsen_US
dc.typeArticleen_US
dc.description.versionFirst author draften_US
pubs.elements-sourcemanual-entryen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Electrical & Computer Engineeringen_US
pubs.publication-statusUnpublisheden_US
dc.identifier.orcid0000-0002-8594-892X (Unlu, M Selim)
dc.identifier.mycv317766


This item appears in the following Collection(s)

Show simple item record