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dc.contributor.authorMailand, Eriken_US
dc.contributor.authorLi, Binen_US
dc.contributor.authorEyckmans, Jeroenen_US
dc.contributor.authorBouklas, Nikolaosen_US
dc.contributor.authorSakar, Mahmut Selmanen_US
dc.date.accessioned2020-04-14T17:19:10Z
dc.date.available2020-04-14T17:19:10Z
dc.date.issued2019-09
dc.identifier.citationErik Mailand, Bin Li, Jeroen Eyckmans, Nikolaos Bouklas, Mahmut Selman Sakar. 2019. "Surface and Bulk Stresses Drive Morphological Changes in Fibrous Microtissues." Biophysical Journal, Volume 117, Issue 5, pp. 975 - 986. https://doi.org/10.1016/j.bpj.2019.07.041
dc.identifier.issn0006-3495
dc.identifier.urihttps://hdl.handle.net/2144/40147
dc.description.abstractEngineered fibrous tissues consisting of cells encapsulated within collagen gels are widely used three-dimensional in vitro models of morphogenesis and wound healing. Although cell-mediated matrix remodeling that occurs within these scaffolds has been extensively studied, less is known about the mesoscale physical principles governing the dynamics of tissue shape. Here, we show both experimentally and by using computer simulations how surface contraction through the development of surface stresses (analogous to surface tension in fluids) coordinates with bulk contraction to drive shape evolution in constrained three-dimensional microtissues. We used microelectromechanical systems technology to generate arrays of fibrous microtissues and robot-assisted microsurgery to perform local incisions and implantation. We introduce a technique based on phototoxic activation of a small molecule to selectively kill cells in a spatially controlled manner. The model simulations, which reproduced the experimentally observed shape changes after surgical and photochemical operations, indicate that fitting of only bulk and surface contractile moduli is sufficient for the prediction of the equilibrium shape of the microtissues. The computational and experimental methods we have developed provide a general framework to study and predict the morphogenic states of contractile fibrous tissues under external loading at multiple length scales.en_US
dc.format.extentp. 975 - 986en_US
dc.languageen
dc.language.isoen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofBiophysical Journal
dc.rights© 2019 Biophysical Society. This work is made available under the Creative Commons Attribution 4.0 International license.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPhysical sciencesen_US
dc.subjectChemical sciencesen_US
dc.subjectBiological sciencesen_US
dc.subjectBiophysicsen_US
dc.titleSurface and bulk stresses drive morphological changes in fibrous microtissuesen_US
dc.typeArticleen_US
dc.description.versionPublished versionen_US
dc.identifier.doi10.1016/j.bpj.2019.07.041
pubs.elements-sourcecrossrefen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0003-1475-8149 (Eyckmans, Jeroen)
dc.identifier.mycv507859


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© 2019 Biophysical Society. This work is made available under the Creative Commons Attribution 4.0 International license.
Except where otherwise noted, this item's license is described as © 2019 Biophysical Society. This work is made available under the Creative Commons Attribution 4.0 International license.