Show simple item record

dc.contributor.authorMansfield, Katelyn M.en_US
dc.contributor.authorCleves, Philip A.en_US
dc.contributor.authorVan Vlack, Emilyen_US
dc.contributor.authorKriefall, Nicola G.en_US
dc.contributor.authorBenson, Brooke E.en_US
dc.contributor.authorCamacho, Dimitrios J.en_US
dc.contributor.authorHemond, Oliviaen_US
dc.contributor.authorPedroza, Moniqueen_US
dc.contributor.authorSiggers, Trevoren_US
dc.contributor.authorPringle, John R.en_US
dc.contributor.authorDavies, Sarah W.en_US
dc.contributor.authorGilmore, Thomas D.en_US
dc.date.accessioned2020-04-15T15:31:09Z
dc.date.available2020-04-15T15:31:09Z
dc.identifier.citationKatelyn Mansfield, Philip Cleves, Emily Van Vlack, Nicola Kriefall, Brooke Benson, Dimitrios Camacho, Olivia Hemond, Monique Pedroza, Trevor Siggers, John Pringle, Sarah Davies, Thomas Gilmore. 2019. "Varied effects of algal symbionts on transcription factor NF-κB in a sea anemone and a coral: possible roles in symbiosis and thermotolerance." bioRxiv, https://doi.org/10.1101/640177
dc.identifier.urihttps://hdl.handle.net/2144/40182
dc.description.abstractMany cnidarians, including the reef-building corals, undergo symbiotic mutualisms with photosynthetic dinoflagellate algae of the family Symbiodiniaceae. These partnerships are sensitive to temperature extremes, which cause symbiont loss and increased coral mortality. Previous studies have implicated host immunity and specifically immunity transcription factor NF-κB as having a role in the maintenance of the cnidarian-algal symbiosis. Here we have further investigated a possible role for NF-κB in establishment and loss of symbiosis in various strains of the anemone Exaiptasia (Aiptasia) and in the coral Pocillopora damicornis. Our results show that NF-κB expression is reduced in Aiptasia larvae and adults that host certain algae strains. Treatment of Aiptasia larvae with a known symbiosis-promoting cytokine, transforming growth factor β, also led to decreased NF-κB expression. We also show that aposymbiotic Aiptasia (with high NF-κB expression) have increased survival following infection with the pathogenic bacterium Serratia marcescens as compared to symbiotic Aiptasia (low NF-κB expression). Furthermore, a P. damicornis coral colony hosting Durusdinium spp. (formerly clade D) symbionts had higher basal NF-κB expression and decreased heat-induced bleaching as compared to two individuals hosting Cladocopium spp. (formerly clade C) symbionts. Lastly, genome-wide gene expression profiling and genomic promoter analysis identified putative NF-κB target genes that may be involved in thermal bleaching, symbiont maintenance, and/or immune protection in P. damicornis. Our results provide further support for the hypothesis that modulation of NF-κB and immunity plays a role in some, but perhaps not all, cnidarian-Symbiodiniaceae partnerships as well as in resistance to pathogens and bleaching.en_US
dc.language.isoen_US
dc.publisherCold Spring Harbor Pressen_US
dc.relation.ispartofbioRxiv
dc.subjectCnidariaen_US
dc.subjectAiptasiaen_US
dc.subjectCoralen_US
dc.subjectNF-κBen_US
dc.subjectImmunityen_US
dc.subjectSymbiosisen_US
dc.titleVaried effects of algal symbionts on transcription factor NF-κB in a sea anemone and a coral: possible roles in symbiosis and thermotoleranceen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1101/640177
pubs.elements-sourcemanual-entryen_US
pubs.notesEmbargo: No embargoen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
pubs.publication-statusPublisheden_US
dc.date.online2019-05-17
dc.identifier.mycv506596


This item appears in the following Collection(s)

Show simple item record