Label-free optical detection of biomolecular translocation through nanopore arrays
Henley, Robert Y.
Toscano, Michael L.
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Citation (published version)Andrey Ivankin, Robert Y Henley, Joseph Larkin, Spencer Carson, Michael L Toscano, Meni Wanunu. 2014. "Label-free optical detection of biomolecular translocation through nanopore arrays.." ACS Nano, Volume 8, Issue 10, pp. 10774 - 10781. https://doi.org/10.1021/nn504551d
In recent years, nanopores have emerged as exceptionally promising single-molecule sensors due to their ability to detect biomolecules at subfemtomole levels in a label-free manner. Development of a high-throughput nanopore-based biosensor requires multiplexing of nanopore measurements. Electrical detection, however, poses a challenge, as each nanopore circuit must be electrically independent, which requires complex nanofluidics and embedded electrodes. Here, we present an optical method for simultaneous measurements of the ionic current across an array of solid-state nanopores, requiring no additional fabrication steps. Proof-of-principle experiments are conducted that show simultaneous optical detection and characterization of ssDNA and dsDNA using an array of pores. Through a comparison with electrical measurements, we show that optical measurements are capable of accessing equivalent transmembrane current information.
Rights"Copyright © 2014 American Chemical Society. This article appears in OpenBU by publisher permission."