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dc.contributor.authorSchaus, Scotten_US
dc.contributor.authorHansen, Ullaen_US
dc.contributor.authorChin, Hang Gyeongen_US
dc.contributor.authorEstève, Pierre Olivieren_US
dc.contributor.authorRuse, Cristianen_US
dc.contributor.authorLee, Jiyoungen_US
dc.contributor.authorPradhan, Sriharsaen_US
dc.date.accessioned2020-05-04T15:35:30Z
dc.date.available2020-05-04T15:35:30Z
dc.date.issued2019-06-10
dc.identifierhttps://www.biorxiv.org/content/10.1101/665984v1
dc.identifier.citationScott Schaus, Ulla Hansen, Hang Gyeong Chin, Pierre Olivier Estève, Cristian Ruse, Jiyoung Lee, Sriharsa Pradhan. "Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8." https://doi.org/10.1101/665984
dc.identifier.urihttps://hdl.handle.net/2144/40531
dc.description.abstractMicrotubules are critical for mitosis, cell motility, and protein and organelle transport, and are a validated target for anticancer drugs. However, tubulin regulation and recruitment in these cellular processes is less understood. Post-translational modifications of tubulin are proposed to regulate microtubule functions and dynamics. Although many such modifications have been investigated, tubulin methylations and enzymes responsible for methylation have only recently begun to be described. Here we report that N-lysine methyl transferase KMT5A (SET8/PR-Set7), which methylates histone H4K20, also methylates α-tubulin. Furthermore, the transcription factor LSF binds both tubulin and SET8, and enhances α-tubulin methylation in vitro, countered by FQI1, a specific small molecule inhibitor of LSF. Thus, the three proteins SET8, LSF, and tubulin, all essential for mitotic progression, interact with each other. Overall, these results point to dual functions for both SET8 and LSF not only in chromatin regulation, but also for cytoskeletal modification.en_US
dc.language.isoen_US
dc.publisherbioRxiven_US
dc.rightsThe copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiochemistryen_US
dc.titleTranscription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8en_US
dc.typeOtheren_US
dc.description.versionFirst author draften_US
dc.identifier.doi10.1101/665984
pubs.elements-sourcemanual-entryen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Chemistryen_US
dc.date.online2019-06-10
dc.identifier.mycv529075


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The copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder. It is made available under a CC-BY 4.0 International license.