Lysosomal machinery drives extracellular acidification to direct non-apoptotic cell death
Ortega, Anthony J.
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Citation (published version)Albert Mondragon, Alla Yalonetskaya, Z.J. Hintze, S. Holmes, Kimberly Mccall. 2019. "Lysosomal machinery drives extracellular acidification to direct non-apoptotic cell death." Cell Reports, Volume 27, pp. 11 - 19. https://doi.org/10.1016/j.celrep.2019.03.034
Cell death is a fundamental aspect of development, homeostasis, and disease; yet, our understanding of non-apoptotic forms of cell death is limited. One such form is phagoptosis, in which one cell utilizes phagocytosis machinery to kill another cell that would otherwise continue living. We have previously identified a non-autonomous requirement of phagocytosis machinery for the developmental programmed cell death of germline nurse cells in the Drosophila ovary; however, the precise mechanism of death remained elusive. Here, we show that lysosomal machinery acting in epithelial follicle cells is used to non-autonomously induce the death of nearby germline cells. Stretch follicle cells recruit V-ATPases and chloride channels to their plasma membrane to extracellularly acidify the germline and release cathepsins that destroy the nurse cells. Our results reveal a role for lysosomal machinery acting at the plasma membrane to cause the death of neighboring cells, providing insight into mechanisms driving non-autonomous cell death.