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dc.contributor.authorKarl, W. Clemen_US
dc.contributor.authorYurdakul, Celalettinen_US
dc.contributor.authorAvci, Oguzhanen_US
dc.contributor.authorMatlock, Alexen_US
dc.contributor.authorDevaux, Alexander J.en_US
dc.contributor.authorQuintero, Maritza V.en_US
dc.contributor.authorOzbay, Ekmelen_US
dc.contributor.authorDavey, Robert A.en_US
dc.contributor.authorConnor, John H.en_US
dc.contributor.authorTian, Leien_US
dc.contributor.authorÜnlü, M. Selimen_US
dc.date2019-10-01
dc.date.accessioned2020-05-11T17:08:56Z
dc.date.available2020-05-11T17:08:56Z
dc.date.issued2020-01-31
dc.identifier.citationWilliam Karl, Celalettin Yurdakul, Oguzhan Avci, Alex Matlock, Alexander Devaux, Maritza Quintero, Ekmel Ozbay, Robert Davey, John Connor, Lei Tian, Selim Unlu. 2020. "High-throughput, high-resolution interferometric light microscopy of biological nanoparticles." ACS Nano, Volume 14, Issue 2, pp. 2002 - 2013. https://doi.org/10.1021/acsnano.9b08512
dc.identifier.urihttps://hdl.handle.net/2144/40747
dc.description.abstractLabel-free, visible light microscopy is an indispensable tool for studying biological nanoparticles (BNPs). However, conventional imaging techniques have two major challenges: (i) weak contrast due to low-refractive-index difference with the surrounding medium and exceptionally small size and (ii) limited spatial resolution. Advances in interferometric microscopy have overcome the weak contrast limitation and enabled direct detection of BNPs, yet lateral resolution remains as a challenge in studying BNP morphology. Here, we introduce a wide-field interferometric microscopy technique augmented by computational imaging to demonstrate a 2-fold lateral resolution improvement over a large field-of-view (>100 × 100 μm2), enabling simultaneous imaging of more than 104 BNPs at a resolution of ∼150 nm without any labels or sample preparation. We present a rigorous vectorial-optics-based forward model establishing the relationship between the intensity images captured under partially coherent asymmetric illumination and the complex permittivity distribution of nanoparticles. We demonstrate high-throughput morphological visualization of a diverse population of Ebola virus-like particles and a structurally distinct Ebola vaccine candidate. Our approach offers a low-cost and robust label-free imaging platform for high-throughput and high-resolution characterization of a broad size range of BNPs.en_US
dc.format.extentp. 2002 - 2013en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.ispartofACS Nano
dc.subjectFourier opticsen_US
dc.subjectBiosensingen_US
dc.subjectComputational imagingen_US
dc.subjectInterference microscopyen_US
dc.subjectLabel-freeen_US
dc.subjectNanoparticle detectionen_US
dc.subjectNanoscience & nanotechnologyen_US
dc.titleHigh-throughput, high-resolution interferometric light microscopy of biological nanoparticlesen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1021/acsnano.9b08512
pubs.elements-sourcemanual-entryen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Electrical & Computer Engineeringen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0002-8770-2886 (Karl, William)
dc.identifier.orcid0000-0002-1316-4456 (Tian, Lei)
dc.identifier.orcid0000-0002-8594-892X (Unlu, Selim)
dc.identifier.mycv514817


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