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dc.contributor.authorBeaulieu, Devin R.en_US
dc.contributor.authorDavison, Ian G.en_US
dc.contributor.authorBifano, Thomas G.en_US
dc.contributor.authorMertz, Jeromeen_US
dc.contributor.authorKılıç, Kıvılcımen_US
dc.date2018-12-20
dc.date.accessioned2020-05-14T19:44:26Z
dc.date.available2020-05-14T19:44:26Z
dc.date.issued2020-02-20
dc.identifierhttps://doi.org/10.1038/s41592-019-0728-9
dc.identifier.citationDevin R Beaulieu, Ian G Davison, Thomas G Bifano, Jerome Mertz, Kıvılcım Kılıç. 2020. "Simultaneous multiplane imaging with reverberation multiphoton microscopy." Nature Methods, Issue March, 2020, https://doi.org/10.1038/s41592-019-0728-9
dc.identifier.urihttps://hdl.handle.net/2144/40888
dc.description.abstractMultiphoton microscopy (MPM) has gained enormous popularity over the years for its capacity to provide high resolution images from deep within scattering samples1. However, MPM is generally based on single-point laser-focus scanning, which is intrinsically slow. While imaging speeds as fast as video rate have become routine for 2D planar imaging, such speeds have so far been unattainable for 3D volumetric imaging without severely compromising microscope performance. We demonstrate here 3D volumetric (multiplane) imaging at the same speed as 2D planar (single plane) imaging, with minimal compromise in performance. Specifically, multiple planes are acquired by near-instantaneous axial scanning while maintaining 3D micron-scale resolution. Our technique, called reverberation MPM, is well adapted for large-scale imaging in scattering media with low repetition-rate lasers, and can be implemented with conventional MPM as a simple add-on.en_US
dc.relation.ispartofNature Methods
dc.subjectAcousticsen_US
dc.subjectAnimalsen_US
dc.subjectBrainen_US
dc.subjectFemaleen_US
dc.subjectImaging, three-dimensionalen_US
dc.subjectMiceen_US
dc.subjectMice, inbred C57BLen_US
dc.subjectMicroscopy, fluorescence, multiphotonen_US
dc.subjectMotor cortexen_US
dc.subjectNeuronsen_US
dc.subjectOlfactory bulben_US
dc.subjectOptics and photonicsen_US
dc.subjectPhantoms, imagingen_US
dc.subjectPhotonsen_US
dc.subjectScattering, radiationen_US
dc.subjectSignal-to-noise ratioen_US
dc.subjectDevelopmental biologyen_US
dc.subjectBiological sciencesen_US
dc.subjectTechnologyen_US
dc.subjectMedical and health sciencesen_US
dc.titleSimultaneous multiplane imaging with reverberation multiphoton microscopyen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1038/s41592-019-0728-9
pubs.elements-sourcemanual-entryen_US
pubs.notes13 pages, 4 figuresen_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Mechanical Engineeringen_US
pubs.publication-statusPublished onlineen_US
dc.date.online2019-12-19
dc.identifier.orcid0000-0003-4952-2515 (Bifano, Thomas G)
dc.identifier.mycv407967


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