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dc.contributor.authorBaumann, Cory W.en_US
dc.contributor.authorKwak, Dongminen_US
dc.contributor.authorFerrington, Deborah A.en_US
dc.contributor.authorThompson, LaDora V.en_US
dc.date.accessioned2020-05-14T19:57:58Z
dc.date.available2020-05-14T19:57:58Z
dc.date.issued2018-07-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000438691200005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e74115fe3da270499c3d65c9b17d654
dc.identifier.citationCory W Baumann, Dongmin Kwak, Deborah A Ferrington, LaDora V Thompson. 2018. "Downhill exercise alters immunoproteasome content in mouse skeletal muscle." Cell Stress & Chaperones, Volume 23, Issue 4, pp. 507 - 517. https://doi.org/10.1007/s12192-017-0857-y
dc.identifier.issn1355-8145
dc.identifier.issn1466-1268
dc.identifier.urihttps://hdl.handle.net/2144/40890
dc.description.abstractContent of the immunoproteasome, the inducible form of the standard proteasome, increases in atrophic muscle suggesting it may be associated with skeletal muscle remodeling. However, it remains unknown if the immunoproteasome responds to stressful situations that do not promote large perturbations in skeletal muscle proteolysis. The purpose of this study was to determine how an acute bout of muscular stress influences immunoproteasome content. To accomplish this, wildtype (WT) and immunoproteasome knockout lmp7-/-/mecl1-/-(L7M1) mice were run downhill on a motorized treadmill. Soleus muscles were excised 1 and 3 days post-exercise and compared to unexercised muscle(control). Ex vivophysiology, histology and biochemical analyses were used to assess the effects of immunoproteasome knockout and unaccustomed exercise. Besides L7M1 muscle being LMP7/MECL1deficient, no other major biochemical, histological or functional differences were observed between the control muscles. In both strains, the downhill run shifted the force-frequency curve to the right and reduced twitch force, however did not alter tetanic force or inflammatory markers. In the days post-exercise, several of the proteasome 's catalytic subunits were upregulated. Specifically, WT muscle increased LMP7 while L7M1 muscle instead increased ≤ 5. These findings indicate that running mice downhill results in subtle contractile characteristics that correspond to skeletal muscle injury, yet does not appear to induce a significant inflammatory response. Interestingly, this minor stress activated the production of specific immunoproteasome subunits; that if knocked out, were replaced by components of the standard proteasome. These data suggest that the immunoproteasome may be involved in maintaining cellular homeostasis.en_US
dc.description.sponsorshipThis study was supported by the Elaine and Robert Larson Endowed Vision Research Chair (to DAF), the National Institutes of Health/National Institute of Aging (T32-AG29796 to CWB), an anonymous benefactor for Macular Degeneration Research, the Lindsay Family Foundation and an unrestricted grant from Research to Prevent Blindness to the Department of Ophthalmology and Visual Neurosciences. The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript. (Elaine and Robert Larson Endowed Vision Research Chair; T32-AG29796 - National Institutes of Health/National Institute of Aging; Lindsay Family Foundation; Research to Prevent Blindness)en_US
dc.format.extentp. 507 - 517en_US
dc.languageEnglish
dc.language.isoen_US
dc.publisherSpringeren_US
dc.relation.ispartofCell Stress & Chaperones
dc.subjectScience & technologyen_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectCell biologyen_US
dc.subjectDegradationen_US
dc.subjectEccentric contractionsen_US
dc.subjectContraction-induced injuryen_US
dc.subjectUbiquitin-proteasome pathwayen_US
dc.subjectDuchenne muscular dystrophyen_US
dc.subjectInduced oxidative stressen_US
dc.subjectLow-frequency fatigueen_US
dc.subjectEccentric exerciseen_US
dc.subjectLengthening contractionsen_US
dc.subjectSelective inhibitoren_US
dc.subjectProtein breakdownen_US
dc.subjectCoupling failureen_US
dc.subjectMuscle functionen_US
dc.subjectOxidative stressen_US
dc.subjectInflammationen_US
dc.subjectMice, inbred C57BLen_US
dc.subjectMuscle, skeletalen_US
dc.subjectOrgan sizeen_US
dc.subjectPhysical conditioning, animalen_US
dc.subjectProtein carbonylationen_US
dc.subjectAnimalsen_US
dc.subjectProteasome endopeptidase complexen_US
dc.subjectExercise testen_US
dc.subjectBody compositionen_US
dc.subjectMuscle contractionen_US
dc.subjectMaleen_US
dc.subjectBiochemistry and cell biologyen_US
dc.subjectBiochemistry & molecular biologyen_US
dc.titleDownhill exercise alters immunoproteasome content in mouse skeletal muscleen_US
dc.typeArticleen_US
dc.description.versionAccepted manuscripten_US
dc.identifier.doi10.1007/s12192-017-0857-y
pubs.elements-sourceweb-of-scienceen_US
pubs.notesEmbargo: No embargoen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent Collegeen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent College, Physical Therapy and Athletic Trainingen_US
pubs.publication-statusPublisheden_US
dc.identifier.mycv291197


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