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dc.contributor.advisorBifano, Thomas G.en_US
dc.contributor.authorBeaulieu, Devin R.en_US
dc.date.accessioned2020-05-19T15:39:05Z
dc.date.available2020-05-19T15:39:05Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/2144/41019
dc.description.abstractMultiphoton microscopy has become an extremely valuable tool for peering deeply into thick, scattering media such as biological tissue. However, the traditional multiphoton beam-scanning approach is held back because only one thin plane is observed at a time. The reverberation loop elegantly overcomes this limitation by generating an infinite series of foci at depths spanning the sample, all sampled individually but near-simultaneously. With the inclusion of some additional interleave steps, it is possible to quickly scan a sample at video rates – allowing volumetric imaging at or near the rate one would traditionally image planes. In neural imaging, this enables a reverberation multiphoton microscope to simultaneously monitor relationships in neuronal activity not only horizontally across samples, but vertically across many layers of the brain. In imaging of engineered cardiac tissues, this enables high resolution observation of three-dimensional structures in a live sample, even as it actively beats and moves.en_US
dc.language.isoen_US
dc.subjectOpticsen_US
dc.titleReverberation multiphoton microscopy for volumetric imaging in scattering mediaen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2020-05-19T04:02:02Z
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineElectrical & Computer Engineeringen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.orcid0000-0003-2336-4352


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