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dc.contributor.advisorSiggers, Trevor Wen_US
dc.contributor.advisorOffner, Gwynneth Den_US
dc.contributor.authorBuckshaw II, Robert S.en_US
dc.date.accessioned2020-06-12T13:43:25Z
dc.date.available2020-06-12T13:43:25Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/2144/41179
dc.description.abstractCoordination of gene expression within the cell requires the integrated actions of various multi-protein, gene regulatory complexes. The Mediator and BRG1 Associate Factor (BAF) complexes are large, dynamic regulatory cofactors (COF) that are made up of multiple different submodules, and play key roles in regulating gene expression. Gene-specific regulation requires that transcription factors (TFs) recruit these COF complexes to gene promoters. How separate subdomains in each complex interact with distinct sets of TFs in each cell remains an important question. In this study, to address this question, we sought to apply the nuclear extract protein-binding microarray (nextPBM) technology being developed in our lab to study interactions between TFs and subunits of the Mediator and BAF complexes. To facilitate this, we cloned, expressed and purified subdomains of proteins from the Mediator and BAF complexes. We then used the nextPBM technology to study the interactions of our subdomains with TFs in human macrophages. We identified several new interactions with TFs, and demonstrate the utility of this approach to student TF-COF interaction.en_US
dc.language.isoen_US
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSystematic biologyen_US
dc.subjectBAFen_US
dc.subjectEpigenetic regulationen_US
dc.subjectImmunologyen_US
dc.subjectMediatoren_US
dc.subjectPBMen_US
dc.subjectSWI/SNFen_US
dc.titleCloning and nextPBM analysis of the mediator and BRG1 associated factor complexesen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2020-06-11T22:01:29Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineMedical Sciencesen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.orcid0000-0002-6000-5853


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International