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dc.contributor.authorGhulman, Motaz A.en_US
dc.date.accessioned2020-11-05T16:26:57Z
dc.date.available2020-11-05T16:26:57Z
dc.date.issued1999
dc.date.submitted1999
dc.identifier.other(OCoLC)1140076399
dc.identifier.urihttps://hdl.handle.net/2144/41599
dc.descriptionPLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.descriptionx, 81 leavesen_US
dc.descriptionThesis (MSD) --Boston University Henry M. Goldman School of Dental Medicine, 1999 (Biomaterials).en_US
dc.descriptionIncludes bibliography: leaves 68-81.en_US
dc.description.abstractThere has been some concern over the estrogenicity of monomers leached from dental composites and sealants. The aim of this study was to test the estrogenic activity of bisglycidyl dimethacrylate (Bis-GMA), bisphenol-A dimethacrylate (Bis-DMA), bisphenol-A (BPA), and triethyleneglycol dimethacrylate (TEGDMA) by an E-SCREEN assay using an estrogen-sensitive MCF-7 cell line model. Unlike BPA, these components may elute from the commercial dental sealants as demonstrated in a recent study (Nathanson et al., 1997). However, their biological effects have been much less studied. Cells were cultured with different concentrations ofthe test components ranging from 10[-12] to 10[-4] M and assayed at 36 and 144 hours. Estradiol-17[beta] was used as a positive control. Sulforhodamine-B (SRB) was used to stain the trichloroacetic acid-fixed cells. The linearity of the SRB assay by cell number was verified and assessed by analysis of variance and an a posteriori Scheffe’s test. The results indicated that at 144 hours, both BPA and Bis-DMA significantly stimulated MCF-7 cell growth by 3.3 and 2.2 fold respectively at 10[-6] M (P<0.05) over the control. A similar increase was observed with a 10[-9] M estradiol concentration whereas Bis-GMA stimulated comparable cell growth at 10[-8] M. Bis-DMA showed estrogenic effects at a concentration as low as 10[-12] M. At higher concentration (10[-4] M), cytotoxicity was observed with all monomers tested. It was concluded that BPA, Bis-DMA and Bis-GMA had an estrogenic effect in this in vitro study whereas TEGDMA did not.en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsThis work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.en_US
dc.subjectPit and fissure sealantsen_US
dc.subjectDental cementsen_US
dc.subjectEstrogensen_US
dc.titleIn vitro estrogenic activity of leachable components from dental sealants and compositesen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Science in Dentistryen_US
etd.degree.levelmastersen_US
etd.degree.disciplineBiomaterialsen_US
etd.degree.grantorBoston Universityen_US


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