Effect of alumina scaffold coated nanocrystal hydroxyapatite on its mechanical properties and cells seeding
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The objectives of this study were designed to test mechanical properties of porous alumina scaffold coated and uncoated with nano hydroxyapatite (n-HA) crystal and the effect of the internal geometry of the porous scaffolds on the cells distribution. The porous alumina scaffolds were made from sponge foam by using polyurethane foam as a template. The prepared foams were immerged in alumina slurry and dried for 3 times before sintering at 1450 degrees C. Alumina scaffolds were divided into two groups: coated and uncoated with nano hydroxyapatite crystals. Normal human osteoblasts were extracted from alveolar bone of young healthy adults, an expanded to the second passage for experiments. Cells were screened for osteoblast phenotype prior to all experiments, seeded onto scaffold blocks at a concentration of 1.5 x 10 cells/cm, and cultured for 16 hours, 7 days, and 14 days. All scaffolds were cut at 0, 3 and 5 millimeters depth. SEM was perfomed at 3 different depth and different time interval. For mechanical testing, and porous alumina coated and uncoated with n-HA were tested by three-point bending test by Instron testing machine. From ANOVA analysis, the result shows that there is no statistically significant difference at p [less than] 0.05 between porous alumina coated with n-HA and porous alumina uncoated with n-HA but dense alumina without resin infusion found that at 1450 degrees C shows the highest strength (9.23MPa). For cells seeding, we found that there are cells penetration on porous alumina scaffold coated and uncoated with n-HA. In porous alumina scaffold coated with n-HA seems to have more potential to stimulate the osteophenotype of cultured human osteoblasts.
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (D.Sc.D.)--Boston University, Goldman School of Dental Medicine, 2008 (Dept. of Restorative Sciences and Biomaterials).Includes bibliographical references: leaves 83-89.
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